Oral biofilms: molecular analysis, challenges, and future prospects in dental diagnostics.
Oral biofilms: molecular analysis, challenges, and future prospects in dental diagnostics.
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DOI：
10.2147/ccide.s31005
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Marsh PD
中科院分区：
文献类型：
--
作者：
Do T;Devine D;Marsh PD
口腔生物膜是功能和结构上有组织的多微生物群落，其包埋在粘膜和牙齿表面上的外聚合物的细胞外基质中。这些生物膜在健康中天然存在，并为宿主提供益处。然而，这种关系可能会破裂，疾病可能会发生;疾病与这些生物膜内物种平衡的变化有关。已经开发了简单的诊断测试，包括选定细菌的培养，例如与龋齿有关的细菌，便于评估个体患者进一步患病的风险。然而，口腔疾病具有复杂的病因学，并且由于目前只有约50%的口腔生物膜可以生长，因此正在开发基于培养的非依赖性分子方法，该方法可以更全面地评估样品中一系列推定病原体的存在。这些生物膜的多样性给研究结果的解释带来了挑战，未来的工作是研究新技术检测口腔生物膜中生物活性和功能的能力，而不仅仅是提供微生物名称的目录。
Oral biofilms are functionally and structurally organized polymicrobial communities that are embedded in an extracellular matrix of exopolymers on mucosal and dental surfaces. These biofilms are found naturally in health, and provide benefits to the host. However, this relationship can break down, and disease can occur; disease is associated with a shift in the balance of the species within these biofilms. Simple diagnostic tests have been developed that involve the culture of selected bacteria, eg, those implicated in dental caries, facilitating an assessment of risk of further disease in individual patients. However, oral diseases have a complex etiology, and because only around 50% of oral biofilm can be grown at present, culture-independent molecular-based approaches are being developed that give a more comprehensive assessment of the presence of a range of putative pathogens in samples. The diversity of these biofilms creates challenges in the interpretation of findings, and future work is investigating the ability of novel techniques to detect biological activity and function in oral biofilms, rather than simply providing a catalogue of microbial names.