Identification of four CCCH zinc finger proteins in Xenopus, including a novel vertebrate protein with four zinc fingers and severely restricted expression
Identification of four CCCH zinc finger proteins in Xenopus, including a novel vertebrate protein with four zinc fingers and severely restricted expression
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DOI：
10.1016/s0378-1119(98)00617-9
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发表时间：
1999-03-04
期刊：
影响因子：
3.5
通讯作者：
Blackshear, PJ
中科院分区：
文献类型：
--
作者：
De, J;Lai, WS;Blackshear, PJ
Tristetraprolin（TTP）是一类CCCH锌指蛋白的原型，是一种在成纤维细胞中由生长因子和血清快速瞬时诱导的磷蛋白。最近的证据表明，TTP的生理功能是通过与其mRNA结合并使其不稳定来抑制肿瘤坏死因子α从巨噬细胞分泌（Carballo，E.，Lai，W.S.，黑剪，P. J.，1998. Science，281，1001-1005）。为了研究CCCH蛋白在非洲爪蟾早期发育中的可能功能，我们分离了四个非洲爪蟾编码该类成员的cDNA。基于49%的总氨基酸同一性和84%的氨基酸同一性内的双锌指结构域，非洲爪蟾蛋白（XC 3 H-1）似乎是TTP的同源物。通过类似的分析，XC 3 H-2和XC 3 H-3是ERF-1（cMG 1，TIS 11B）和ERF-2（TIS 11 D）的同源物。第四种蛋白XC 3 H-4是先前未鉴定的脊椎动物锌指蛋白CCCH类成员;它含有四个Cx（8）Cx（5）Cx（3）H重复序列，其中两个是YKTEL Cx（8）Cx（5）Cx（3）H重复序列，其与在其它CCCH蛋白中发现的序列密切相关，而XC 3 H-1、XC 3 H-2和XC 3 H-3在成体组织中广泛表达，XC 3 H-4 mRNA在除卵巢外的任何成体组织中均未检测到。其表达似乎仅限于卵巢、卵母细胞、卵和早期胚胎阶段，直至囊胚中期过渡。其mRNA在各年龄组卵母细胞中均高表达，并在成熟卵母细胞的极细胞质中富集。母体表达也与其他三个信息，这表明这些蛋白质参与调节卵母细胞成熟和/或早期胚胎发生中的mRNA稳定性的可能性。(C)1999 Elsevier Science B. V.保留所有权利。
Tristetraprolin (TTP, the prototype of a class of CCCH zinc finger proteins, is a phosphoprotein that is rapidly and transiently induced by growth factors and serum in fibroblasts. Recent evidence suggests that a physiological function of TTP is to inhibit tumor necrosis factor alpha secretion from macrophages by binding to and destabilizing its mRNA (Carballo, E., Lai, W.S., Blackshear, P.J., 1998. Science, 281, 1001-1005). To investigate possible functions of CCCH proteins in early development of Xenopus, we isolated four Xenopus cDNAs encoding members of this class. Based on 49% overall amino acid identity and 84% amino acid identity within the double zinc finger domain, one of the Xenopus proteins (XC3H-1) appears to be the homologue of TTP. By similar analyses, XC3H-2 and XC3H-3 are homologues of ERF-1 (cMG1, TIS11B) and ERF-2 (TIS11D). A fourth protein, XC3H-4, is a previously unidentified member of the CCCH class of vertebrate zinc finger proteins; it contains four Cx(8)Cx(5)Cx(3)H repeats, two of which are YKTEL Cx(8)Cx(5)Cx(3)H repeats that are closely related to sequences found in the other CCCH proteins, Whereas XC3H-1, XC3H-2, and XC3H-3 were widely expressed in adult tissues, XC3H-4 mRNA was not detected in any of the adult tissues studied except for the ovary. Its expression appeared to be limited to the ovary, oocyte, egg and the early embryonic stages leading up to the mid-blastula transition. Its mRNA was highly expressed in oocytes of all ages, and was enriched in the animal pole cytosol of mature oocytes. Maternal expression was also seen with the other three messages, suggesting the possibility that these proteins are involved in regulating mRNA stability in oocyte maturation and/or early embryogenesis. (C) 1999 Elsevier Science B.V. All rights reserved.