Expression and location of phospho-Artemis (Serine516) in hair follicles during induced growth of mouse hair
Expression and location of phospho-Artemis (Serine516) in hair follicles during induced growth of mouse hair
复制标题
小鼠毛发诱导生长过程中毛囊中磷酸-Artemis (Serine516) 的表达和定位
DOI：
10.1007/s00403-012-1233-5
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发表时间：
2012-05-01
影响因子：
3
通讯作者：
Zheng, Min
中科院分区：
文献类型：
--
作者：
Wu, Xian-Jie;Zhu, Jian-Wei;Zheng, Min
Artemis 被认为在 NHEJ 中发挥作用，而且它也是一种多功能蛋白质。先前的研究发现，由于 Artemis 突变导致 Omenn 综合征样表型，并与脱发相关。由于 Artemis 的 C 末端（包括 Serine516）磷酸化是 Artemis 核酸内切酶反应的先决条件，因此我们推测 Artemis (Serine516) 可能在毛囊中表达并与毛发周期相关。在这项研究中，通过拔掉背部的休止期毛发来诱导 C57BL/6 小鼠的毛发生长。使用冷冻切片和特异性多克隆抗 Artemis (Serine516) 免疫球蛋白 G (IgG) 抗体，通过免疫荧光评估毛发生长周期期间毛囊中 Artemis (Serine516) 的表达。在休止期邻近表皮的生殖细胞、帽子和球状毛发中检测到了它。在生长期 II 中，在整个毛囊间表皮和角化细胞链中发现了 Artemis (Serine516) 的强烈染色。在生长期 IV 中，在基底细胞以及外根鞘 (ORS) 和内根鞘 (IRS) 的上部检测到 Artemis (Serine516) 的强烈染色。但在生长期 VI 中，只有上部 ORS 和下部髓质染色呈阳性。上ORS和下皮质在退行期用Artemis (Serine516)呈阳性染色。基于Artemis (Serine516)在毛发生长中期和成熟毛发生长初期的表达强于毛发生长终期和毛发生长中期的现象，我们推测它可能在小鼠毛发的诱导生长中发挥作用。
Artemis has been implicated in having a role in NHEJ, and it is also a multifunctional protein. Previous studies have found Omenn syndrome-like phenotype due to Artemis mutations and associated with alopecia. As Artemis phosphorylation in its c-terminus including Serine516 is prerequisite for the Artemis endonuclease reaction, we postulate that Artemis (Serine516) may be expressed in hair follicle and relate to hair cycling. In this study, hair growth in C57BL/6 mice was induced by plucking the telogen hair on the back. Expression of Artemis (Serine516) in hair follicles during the hair growth cycle was evaluated by immunofluorescence using cryosections and a specific polyclonal anti-Artemis (Serine516) immunoglobulin G (IgG) antibody. It was detected in germ cells, cap, and club hair adjoining the epidermis in telogen. In anagen II, intense staining for Artemis (Serine516) was found in the whole interfollicular epidermis, and in strand keratinocytes. In anagen IV, intense staining for Artemis (Serine516) was detected in basal cells and upper of outer root sheath (ORS) and inner root sheath (IRS). But only upper ORS and lower medulla were stained positive in anagen VI. Upper ORS and lower cortex were positively stained with Artemis (Serine516) in catagen. Based on the phenomenon that the expression of Artemis (Serine516) in mid-anagen and mature anagen was stronger than that in telogen and catagen, we suggest it may take roles in induced growth of mouse hair.