Partial amino acid sequences of botulinum neurotoxins types B and E.
Partial amino acid sequences of botulinum neurotoxins types B and E.
复制标题
B 型和 E 型肉毒杆菌神经毒素的部分氨基酸序列。
DOI：
10.1016/0003-9861(85)90198-5
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发表时间：
1985
影响因子：
3.9
通讯作者：
DasGupta,BR
中科院分区：
文献类型：
--
作者：
Schmidt,JJ;Sathyamoorthy,V;DasGupta,BR
肉毒梭菌E型神经毒素是一种分子量为147，000的单链蛋白，经纯化后进行氨基酸序列测定。对单链肉毒杆菌B型神经毒素（Mr 152，000）以及通过有限胰蛋白酶消化从B型衍生的重链和轻链（分别为Mr 104，000和51，000）进行相同的操作。鉴定了12 - 18个残基，并得出以下结论：（1）有切口（双链）B型轻链衍生自单链（无切口）亲本神经毒素的N-末端三分之一;（2）单链B和E型与有切口A型轻链之间存在序列同源性[J. J.施密特、V. Sathyamoorthy和B. R. 03 The Dog（1984）Res.Commun.119，900-904];（3）A型和B型重链的N-末端区域具有一些结构相似性;和（4）B型神经毒素的活化不涉及从N-末端去除氨基酸或肽。
Clostridium botulinumtype E neurotoxin, a single-chain protein ofMr147,000, was purified and subjected to amino acid sequencing. The same was done for single-chain botulinum type B neurotoxin (Mr152,000), and for the heavy and light chains (Mr104,000 and 51,000 respectively) derived from type B by limited trypsin digestion. Twelve to eighteen residues were identified and the following conclusions were drawn: (1) The light chain of the nicked (dichain) type B is derived from the N-terminal one-third of the single-chain (unnicked) parent neurotoxin; (2) sequence homologies are present between single-chain types B and E and the light chain of the nicked type A [J. J. Schmidt, V. Sathyamoorthy, and B. R. DasGupta (1984)Biochem. Biophys. Res. Commun.119, 900–904]; (3) the N-terminal regions of the heavy chains of types A and B have some structural similarity; and (4) activation of type B neurotoxin cannot involve removal of amino acids or peptides from the N terminus.