Immunogenicity of self-adjuvanticity oral vaccine candidate based on use of Bacillus subtilis spore displaying Schistosoma japonicum 26 KDa GST protein
Immunogenicity of self-adjuvanticity oral vaccine candidate based on use of Bacillus subtilis spore displaying Schistosoma japonicum 26 KDa GST protein
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基于使用展示日本血吸虫 26 KDa GST 蛋白的枯草芽孢杆菌孢子的自身佐剂口服候选疫苗的免疫原性
DOI：
10.1007/s00436-009-1606-7
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发表时间：
2009-11-01
影响因子：
2
通讯作者：
Yu, Xinbing
中科院分区：
文献类型：
--
作者：
Li, Li;Hu, Xuchu;Yu, Xinbing
粘膜免疫的一种有前途的方法依赖于活的重组疫苗载体。在这项研究中，我们使用了六个胞外蛋白酶缺陷的枯草芽孢杆菌菌株WB 600表达日本血吸虫26 kDa谷胱甘肽S-转移酶（GST）。用Western blot、免疫荧光和流式细胞术分析SjGST在孢子表面的表达。SjGST重组孢子用于小鼠口服疫苗接种，并显示产生粘膜和全身反应。口服给药后第33天，粪便中SjGST特异性分泌伊加和血清中IgG均显著增加。重组S.日本血吸虫SjGST在B.枯草杆菌WB 600孢子具有良好的免疫原性，而B.枯草芽孢杆菌孢子可作为潜在的粘膜递送载体，为未来的寄生虫预防和控制提供更有效的疫苗接种策略。
One of the promising approaches in mucosal immunization relies on live recombinant vaccine carriers. In this study, we used a six-extracellular protease-deficient Bacillus subtilis strain WB600 to express Schistosoma japonicum 26 kDa glutathione S-transferase (GST). Western blot, immunofluorescence, and flow cytometry analyses were used to identify SjGST expression on spore surface. SjGST recombinant spores were used for oral vaccination in mice and were shown to generate mucosal and systemic response. Both SjGST-specific secretory IgA in feces and IgG in serum augmented significantly on day 33 after oral administration. It seemed that surface display of recombinant S. japonicum SjGST on B. subtilis WB600 spores showed good immunogenicity, and B. subtilis spores could be used as potential mucosal delivery vehicles to provide more effective vaccination strategies for parasite prevention and control in the future.