A plant mitochondrial sequence transcribed in transgenic tobacco chloroplasts is not edited.
A plant mitochondrial sequence transcribed in transgenic tobacco chloroplasts is not edited.
复制标题
转基因烟草叶绿体中转录的植物线粒体序列未经编辑。
DOI：
10.1128/mcb.15.3.1377
复制
发表时间：
1995
影响因子：
5.3
通讯作者：
Maliga,P
中科院分区：
文献类型：
--
作者：
Sutton,CA;Zoubenko,OV;Hanson,MR;Maliga,P
RNA editing occurs in two higher-plant organelles, chloroplasts and mitochondria. Because chloroplasts and mitochondria exhibit some similarity in editing site selection, we investigated whether mitochondrial RNA sequences could be edited in chloroplasts. We produced transgenic tobacco plants that contained chimeric genes in which the second exon of aPetunia hybridamitochondrialcoxIIgene was under the control of chloroplast gene regulatory sequences.coxIItranscripts accumulated to low or high levels in transgenic chloroplasts containing chimeric genes with the plastid ribosomal protein generps16or the rRNA operon promoter, respectively. Exon 2 ofcoxIIwas chosen because it carries seven editing sites and is edited in petunia mitochondria even when located in an abnormal context in an aberrant recombined gene. When editing of thecoxIItranscripts in transgenic chloroplasts was examined, no RNA editing at any of the usual sites was detected, nor was there any novel editing at any other sites. These results indicate that the RNA editing mechanisms of chloroplasts and mitochondria are not identical but must have at least some organelle-specific components.
