The relation of the multifocal electroretinographic response to macular layer volume.
The relation of the multifocal electroretinographic response to macular layer volume.
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多焦视网膜电图反应与黄斑层体积的关系。
DOI：
10.1007/s10633-022-09873-z
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Akula,JamesD
中科院分区：
文献类型：
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作者：
Fonseca,MarianaI;Nouck-A-Nwal,Alexandra;Ambrosio,Lucia;Altschwager,Pablo;Hansen,RonaldM;Fulton,AnneB;Akula,JamesD
目的探讨多焦视网膜电图（mfERG）反应幅度与各mfERG元素刺激区域内视网膜层、后受体层和光感受器层体积的关系。方法选取16名健康青年作为对照。标准的缩放mfERG的103个六边形元素中的每一个都与使用光学相干断层扫描成像的视网膜斑块对齐。排除落在中央窝和视神经头上的刺激。然后使用线性混合效应建模来推导整个80毫秒标准时间内mfERG响应的估计系数（电压/体积）。然后将每一层产生的预测反应幅度与从其他猴子眼睛研究中获得的药理学“解剖”的mferg进行比较。结果在整个反应过程中，(1)内层视网膜的模型贡献幅度小到中等，(2)后感受器视网膜更大，包含两个突出的峰，(3)光感受器反应最大，与整体（即完整）反应最接近，包括晚出现的振荡。当各层响应的绝对幅值最大时，各层贡献的意义最大。在完整反应的突出波谷和波峰之间的间隔中，内视网膜的贡献是最显著的。后感受器和光感受器反应的贡献在完整反应的突出波谷和波峰中最为显著。结论该模型的结果与先前关于细胞对mfERG的贡献的解释基本一致。这与药理学解剖的猴子mfERG反应也相当一致。因此，视网膜层对mfERG的贡献的估计似乎是可信的。
PurposeTo determine the association of the multifocal electroretinographic (mfERG) response amplitude with the volumes of theinner,postreceptor, andphotoreceptorretinal layers in the region stimulated by each mfERG element.MethodsSixteen healthy, young adult control subjects were studied. Each of the 103 hexagonal elements of the standard, scaled mfERG were aligned, where possible, with patches of retina imaged using optical coherence tomography. Stimuli falling on the fovea and on the optic nerve head were excluded. Linear mixed-effects modeling was then used to derive estimated coefficients (voltage/volume) for the mfERG response throughout the full 80 ms standard epoch. The resulting predicted response amplitudes originating in each layer were then compared to pharmacologically “dissected” mfERGs obtained from other studies in monkey eyes.ResultsAcross the duration of the response, the amplitude of the modeled contribution from (1) theinnerretina was small-to-modest, (2) thepostreceptorretina was larger and contained two prominent peaks, and (3) thephotoreceptorresponse was the largest and most closely paralleled the overall (i.e., intact) response, including late-appearing oscillations. The significance of each layer’s contribution was greatest when the absolute amplitude of that layer’s response was largest. The contribution of the inner retina was maximally significant in the intervalbetweenthe prominent troughs and peaks of the intact response. The contributions of the postreceptor and photoreceptor responses were maximally significantatthe prominent troughs and peaks of the intact response.ConclusionsThe results of the model were in good overall agreement with previous interpretations of the cellular contributions to the mfERG. There was also fair agreement with pharmacologically dissected monkey mfERG responses. Thus, the estimations of the contributions of the retinal layers to the mfERG so produced appeared plausible.