Peptide-rich venom from the spider Heteropoda venatoria potently inhibits insect voltage-gated sodium channels
Peptide-rich venom from the spider Heteropoda venatoria potently inhibits insect voltage-gated sodium channels
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来自蜘蛛 Heteropoda venatoria 的富含肽的毒液可有效抑制昆虫电压门控钠通道
DOI：
10.1016/j.toxicon.2016.11.252
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发表时间：
2017-01-01
期刊：
影响因子：
2.8
通讯作者：
Liu, Zhonghua
中科院分区：
文献类型：
--
作者：
Huang, Yazhou;Wu, Xinzhou;Liu, Zhonghua
Heteropoda venatoria是一种分布于世界各地的有毒蜘蛛，具有以昆虫为食的特征。反相高效液相色谱和基质辅助激光解吸/电离飞行时间质谱分析表明，H。venatoria毒液含有数百种多肽，主要分子量为3000-5000 Da，腹腔注射毒液对蟑螂有严重的毒性作用，在较高浓度下可导致死亡。对蟑螂的LD_（50）为28.18 μ g/g体重。结果发现，该毒液对美洲大蠊背侧不成对正中（DUM）神经元的电压门控钠通道（VGSC）具有强效抑制作用，IC 50值为6.25 +/- 0.02 μ g/mL。然而，100 μ g/mL毒液仅部分阻断大鼠背根神经节细胞的VGSC电流，抑制作用远低于DUM VGSC。结果表明，H. venatoria含有多种神经毒素，可能成为生物杀虫剂的新线索。(C)2016爱思唯尔有限公司版权所有
Heteropoda venatoria is a venomous spider species distributed worldwide and has a characteristic habit of feeding on insects. Reverse-phase high-performance liquid chromatography and matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry analyses revealed that H. venatoria venom contains hundreds of peptides with a predominant molecular weights of 3000-5000 Da. Intra-abdominal injection of the venom had severe toxic effects on cockroaches and caused death at higher concentrations. The LD50 was 28.18 mu g/g of body weight in the cockroach. It was found that the venom had potent inhibitory effect on voltage-gated sodium channels (VGSCs) in Periplaneta americana cockroach dorsal unpaired median (DUM) neurons with an IC50 values of 6.25 +/- 0.02 mu g/mL. However, 100 mu g/mL venom only partially blocked VGSC currents in rat dorsal root ganglion cells, a much lower inhibitory effect than that on DUM VGSCs. Our results indicate that the venom of H. venatoria contains diverse neurotoxins that might become new leads for bioinsecticides. (C) 2016 Elsevier Ltd. All rights reserved.