An Arabidopsis gene encoding a C2H2-domain protein with alternatively spliced transcripts is essential for endosperm development
An Arabidopsis gene encoding a C2H2-domain protein with alternatively spliced transcripts is essential for endosperm development
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编码具有可变剪接转录本的 C2H2 结构域蛋白的拟南芥基因对于胚乳发育至关重要。
DOI：
10.1093/jxb/ers243
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发表时间：
2012-10-01
影响因子：
6.9
通讯作者：
Zhang, Chunyi
中科院分区：
文献类型：
--
作者：
Lu, Xiaoduo;Li, Yuan;Zhang, Chunyi
胚乳是种子的重要组成部分，它维持胚胎发育并包含储存储备。胚乳发育涉及在没有胞质分裂的情况下进行的一系列核分裂。在这项研究中，表明携带破坏的At4g24900基因的突变拟南芥植物在种子发育过程中表现出严重的缺陷。 At4g24900 编码一种核定位的含有 C2H2 的蛋白质，并且该基因的转录物被选择性剪接，从而产生至少九种差异剪接亚型。该基因中的杂合T-DNA插入突变导致25%的种子败育，纯合突变等位基因表现出胚胎致死性。微分干涉对比（DIC）分析显示，该突变体的胚乳核具有多个核仁，并且胚胎在球状到心状阶段的转变中停滞。由于该突变体表现出类似泰坦的表型，因此将其命名为 ttl。 TTL 基因优先在细胞快速分裂的组织中表达，如 P(TTL)::GUS（β-葡萄糖醛酸酶）转基因植物所示。有人提出，TTL 可能是胚乳核分裂的关键调节因子。
The endosperm is an essential part of the seed which sustains embryo development and contains storage reserves. Endosperm development involves a series of nuclear divisions in the absence of cytokinesis. In this study, it is shown that a mutant Arabidopsis plant carrying the disrupted At4g24900 gene exhibits severe defects during seed development. At4g24900 encodes a nuclear-localized C2H2-containing protein and the transcripts of this gene are alternatively spliced, consequently producing at least nine differentially spliced isoforms. The heterozygous T-DNA insertion mutation in this gene resulted in abortion of 25% of seeds, and the homozygous mutant allele displayed embryo lethality. Differential interfernce contrast (DIC) analyses revealed that the mutant has endosperm nuclei with more than one nucleolus and an embryo arrested at the globular to heart stage transition. Because this mutant exhibits a titan-like phenotype, it was designated ttl. The TTL gene is preferentially expressed in tissues with quickly dividing cells as revealed in P(TTL)::GUS (β-glucuronidase) transgenic plants. It is proposed that TTL is likely to function as a key regulator of endosperm nuclear division.