Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development
Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development
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DOI：
10.1038/nn1752
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发表时间：
2006-09-01
影响因子：
25
通讯作者：
Osten, Pavel
中科院分区：
文献类型：
--
作者：
Komai, Shoji;Licznerski, Pawel;Osten, Pavel
感觉体验对于正常的皮层发育是必要的。这已经通过感觉剥夺和皮层的药理学扰动来证明。由于这些操作影响整个皮层网络，突触后细胞特性在经验依赖性发育中的作用尚不清楚。在这里，我们解决了发展中的作用，体树突兴奋性，使突触后尖峰时间依赖形式的可塑性，在大鼠体感皮层。我们使用短干扰RNA（siRNA）为基础的敲低Na+通道，以抑制体树突的兴奋性的少数2/3层锥体神经元在桶皮质，而不改变上升的感觉通路。来自siRNA表达细胞的体内记录显示，这种操作干扰了感觉反应的正常发育加强。邻近皮层神经元的感觉反应性没有变化，表明皮层网络没有变化。我们的结论是体树突兴奋性的突触后神经元的突触强度的调节所需的发展中的感觉皮层。
Sensory experience is necessary for normal cortical development. This has been shown by sensory deprivation and pharmacological perturbation of the cortex. Because these manipulations affect the cortical network as a whole, the role of postsynaptic cellular properties during experience-dependent development is unclear. Here we addressed the developmental role of somatodendritic excitability, which enables postsynaptic spike timing-dependent forms of plasticity, in rat somatosensory cortex. We used short interfering RNA (siRNA)-based knockdown of Na+ channels to suppress the somatodendritic excitability of small numbers of layer 2/3 pyramidal neurons in the barrel cortex, without altering the ascending sensory pathway. In vivo recordings from siRNA-expressing cells revealed that this manipulation interfered with the normal developmental strengthening of sensory responses. The sensory responsiveness of neighboring cortical neurons was unchanged, indicating that the cortical network was unchanged. We conclude that somatodendritic excitability of the postsynaptic neuron is needed for the regulation of synaptic strength in the developing sensory cortex.