The index influenza A virus subtype H5N1 isolated from a human in 1997 differs in its receptor-binding properties from a virulent avian influenza virus.
The index influenza A virus subtype H5N1 isolated from a human in 1997 differs in its receptor-binding properties from a virulent avian influenza virus.
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DOI：
10.1099/vir.0.19519-0
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发表时间：
2004-04
期刊：
影响因子：
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通讯作者：
K. Iwatsuki-Horimoto;Rie Kanazawa;S. Sugii;Y. Kawaoka;T. Horimoto
中科院分区：
文献类型：
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作者：
K. Iwatsuki-Horimoto;Rie Kanazawa;S. Sugii;Y. Kawaoka;T. Horimoto
为了深入了解禽流感病毒传播给人类时发生的事件，通过使用血细胞吸附试验，比较了1997年从人身上分离的H5 N1流感病毒和A/turkey/安大略/7732/66（H5 N9）病毒的受体结合特性。表达人分离株血凝素（HA）的细胞被鸡红细胞（RBC）和人RBC吸附;表达禽病毒HA的细胞仅被鸡RBC吸附。这些结果表明，人类和禽流感病毒H5 HA在识别不同动物物种RBC上的唾液酸寡糖方面存在差异。突变分析表明，寡糖链和HA受体结合位点周围的氨基酸序列的差异是造成这种受体结合差异的原因。这些数据进一步支持了受体识别的改变对于禽流感病毒在人体内的复制很重要的概念。
To gain insight into the events that occur when avian influenza viruses are transmitted to humans, the receptor-binding properties of the index H5N1 influenza virus isolated from a human in 1997 and the A/turkey/Ontario/7732/66 (H5N9) virus were compared, by using a haemadsorption assay. Cells expressing the haemagglutinin (HA) of the human isolate were adsorbed by both chicken red blood cells (RBCs) and human RBCs; those expressing the avian virus HA were only adsorbed by chicken RBCs. These results indicate that human and avian influenza virus H5 HAs differ in their recognition of sialyloligosaccharides on the RBCs of different animal species. Mutational analyses indicated that differences in both the oligosaccharide chains and in the amino acid sequences around the HA receptor-binding site were responsible for this difference in receptor binding. These data further support the concept that alteration in receptor recognition is important for replication of avian viruses in humans.