Exciton energy transfer-based fluorescent sensing through aptamer-programmed self-assembly of quantum dots.
Exciton energy transfer-based fluorescent sensing through aptamer-programmed self-assembly of quantum dots.
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DOI：
10.1021/ac403023p
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发表时间：
2013-11
影响因子：
7.4
通讯作者：
Jianbo Liu;Yan Liu;Xiaohai Yang;Kemin Wang;Qing Wang;Hui Shi;Li Li-Li
中科院分区：
文献类型：
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作者：
Jianbo Liu;Yan Liu;Xiaohai Yang;Kemin Wang;Qing Wang;Hui Shi;Li Li-Li
利用分裂适体编程的量子点自组装技术，建立了一种基于激子能量转移的超灵敏荧光检测生物小分子和蛋白质的新策略。信号是由自组装量子点的激子能量传递产生的。识别是使用适配体传感器支架设计的两个分裂片段序列，具体结合到模型分析物。由分析物触发的量子点自组装引起的粒子组装程度导致粒子间的激子能量转移效应，产生易于检测的荧光猝灭和发射峰的红移，这使我们能够在双信号模式下量化目标。该技术的应用很好地证明了使用两个代表性的分裂适配体为基础的模型系统检测腺苷和凝血酶。这种基于激子能量转移的荧光传感灵敏度远远优于基于等离子体耦合的比色方法。腺苷和凝血酶的检出限（lod）分别可达12 nM和15 pM。该传感策略被提出作为鲁棒性和特异性适配体-目标分析的通用平台，可以进一步发展以监测广泛的目标分析物。本研究提出的概念和方法在分子结合事件的生物学和医学应用研究中具有良好的前景。
A novel exciton energy transfer-based ultrasensitive fluorescent sensing strategy for the detection of biological small molecules and protein has been established through split aptamer-programmed self-assembly of quantum dots (QDs). The signal is produced from exciton energy transfer of the self-assembled QDs. The recognition is accomplished using an aptamer sensor scaffold designed with two split fragment sequences, which specifically bind to the model analytes. The extent of particle assembly, induced by the analyte-triggered self-assembly of QDs, leads to an exciton energy transfer effect between interparticles, giving a readily detectable fluorescent quenching and red shift of the emission peak, which enables us to quantitate the target in dual signal modes. The application of the technique is well demonstrated using two representative split aptamer-based model systems for the detection of adenosine and thrombin. The sensitivity of this exciton energy transfer-based fluorescent sensing is much better than that of plasmonic coupling-based colorimetric methods. Limit of detections (LODs) down to 12 nM and 15 pM can be achieved for adenosine and thrombin, respectively. The sensing strategy is proposed as a general platform for robust and specific aptamer-target analysis which could be further developed to monitor a wide range of target analytes. The concept and methodology developed in this work shows a good promise in the study of molecular binding events in the biological and medical applications.