Extremely low genetic diversity in the European clade of the model bryophyte Anthoceros agrestis
Extremely low genetic diversity in the European clade of the model bryophyte Anthoceros agrestis
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DOI：
10.1007/s00606-020-01676-6
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发表时间：
2020-04-04
影响因子：
1.9
通讯作者：
Forrest, Laura L.
中科院分区：
文献类型：
--
作者：
Dawes, Thomas N.;Villarreal, Juan Carlos A.;Forrest, Laura L.
金鱼藻是一种研究共生相互作用和固碳过程的模式系统。它是一个一年生物种，基因组非常小和紧凑。该植物的单一种质已被证明与世界各地的多年生金鱼草花椒有亲缘关系。我们首次对这两个物种的进化史进行了详细的洞察。由于细胞器基因座的保守性，我们对花椒A的多个种质进行了测序。斑点状复合体使用三个核区：核糖体间隔区ITS2，以及来自单拷贝编码基因rbcs和光敏色素的外显子和内含子区域。我们使用系统发育和年代分析来揭示这两个分类群之间的关系。我们的分析解析了一个遗传上接近一致的欧洲黑麦草种质谱系和两个非欧洲黑麦草谱系。此外，世界性物种斑点花椒形成了两个谱系，一个主要来自欧洲，另一个来自印度。所有被研究的欧洲A.agrestis种质都有一个单一的来源，辐射时间相对较近(不到100万年前)，目前严格与农业生态系统生境有关。
The hornwort Anthoceros agrestis is emerging as a model system for the study of symbiotic interactions and carbon fixation processes. It is an annual species with a remarkably small and compact genome. Single accessions of the plant have been shown to be related to the cosmopolitan perennial hornwort Anthoceros punctatus. We provide the first detailed insight into the evolutionary history of the two species. Due to the rather conserved nature of organellar loci, we sequenced multiple accessions in the Anthoceros agrestis-A. punctatus complex using three nuclear regions: the ribosomal spacer ITS2, and exon and intron regions from the single-copy coding genes rbcS and phytochrome. We used phylogenetic and dating analyses to uncover the relationships between these two taxa. Our analyses resolve a lineage of genetically near-uniform European A. agrestis accessions and two non-European A. agrestis lineages. In addition, the cosmopolitan species Anthoceros punctatus forms two lineages, one of mostly European accessions, and another from India. All studied European A. agrestis accessions have a single origin, radiated relatively recently (less than 1 million years ago), and are currently strictly associated with agroecosystem habitats.