Fucoidan, a major component of brown seaweed, prohibits the growth of human cancer cell lines in vitro.
Fucoidan, a major component of brown seaweed, prohibits the growth of human cancer cell lines in vitro.
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褐藻糖胶是褐藻的主要成分，可在体外抑制人类癌细胞系的生长。
DOI：
10.3892/mmr.1.4.537
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发表时间：
2008
影响因子：
3.4
通讯作者：
M. Kojiro
中科院分区：
文献类型：
--
作者：
S. Fukahori;H. Yano;J. Akiba;S. Ogasawara;S. Momosaki;S. Sanada;Kei Kuratomi;Yasuhiro Ishizaki;Fukuko Moriya;M. Yagi;M. Kojiro
岩藻依聚糖硫酸酯是硫酸化多糖的总称，具有抗肿瘤、抗凝血和抗病毒等多种生物活性。虽然已经对其进行了研究，但其抗肿瘤作用的机制仍然不清楚。本研究采用MTT法检测了从冲绳mozuku中提取的褐藻多糖硫酸酯对15种人癌细胞系（6种肝细胞癌、1种胆管癌、1种胆囊癌、2种卵巢癌、1种肝母细胞瘤、1种神经母细胞瘤和3种肾癌）的抗肿瘤作用。流式细胞仪检测细胞凋亡和细胞周期的变化。结果显示，细胞增殖抑制在13个细胞系中的时间和/或剂量依赖性的方式，这种抑制是显着的肝细胞癌，胆管癌和胆囊癌细胞系。相反，神经母细胞瘤和2个卵巢癌细胞系中的1个的增殖不受影响。6株肝癌细胞系中有5株凋亡细胞比例明显增高，3株G2/M期细胞比例增高。这些观察结果表明，褐藻糖胶是一种潜在的抗肿瘤剂，用于治疗胆管癌，如肝细胞癌，胆管癌和胆囊癌。
Fucoidan, the general term for sulfated polysaccharides, is reported to engage in various biological activities having anti-tumor, anti-coagulation and anti-viral effects. Though it has been investigated, the mechanism of its anti-tumor effects remains elusive. The current study examined the anti-tumor effects of fucoidan extracted from Okinawa mozuku on 15 human cancer cell lines (6 hepatocellular carcinomas, 1 cholangiocarcinoma, 1 gallbladder cancer, 2 ovarian cancers, 1 hepatoblastoma, 1 neuroblastoma and 3 renal cancers) using an MTT assay. Changes in apoptosis and the cell cycle were analyzed by flow cytometry. The results revealed that cell proliferation was suppressed in 13 cell lines in a time- and/or dose-dependent manner; this suppression was marked in the hepatocellular carcinoma, cholangiocarcinoma and gallbladder carcinoma cell lines. In contrast, proliferation of the neuroblastoma and 1 of the 2 ovarian carcinoma cell lines was not affected. The ratio of apoptotic cells significantly increased in 5 of the 6 hepatocellular carcinoma cell lines, and the ratio of G2/M cells increased in the 3 hepatocellular cell lines examined. These observations indicate that fucoidan is a potential anti-tumor agent for the treatment of bile duct cancers, such as hepatocellular carcinoma, cholangiocarcinoma and gall-bladder carcinoma.