Exercise stimulates neovascularization in occluded muscle without affecting bFGF content.
Exercise stimulates neovascularization in occluded muscle without affecting bFGF content.
复制标题
运动刺激闭塞肌肉中的新血管形成，而不影响 bFGF 含量。
DOI：
10.1097/00005768-199911000-00016
复制
发表时间：
1999
影响因子：
4.1
通讯作者：
Ogilvie,RW
中科院分区：
文献类型：
--
作者：
Deschenes,MR;Ogilvie,RW
目的：本研究旨在确定运动诱导的外周动脉功能不全（PAI）肌肉毛细血管改善是否伴随内皮细胞有丝分裂，以及这种反应是否可以通过碱性成纤维细胞生长因子（bFGF）表达的变化来解释。bFGF是一种已知的有丝分裂原。方法双侧股动脉结扎后，Sprague-Dawley大鼠要么静止不动，要么参加跑步机跑步方案。记录每个会话的运行时间到耗尽为止。实验第1、2、3、5、7天，分别对训练结扎动物和静止结扎动物实施安乐死，取腓肠肌段。通过组织化学测定毛细血管的细度，并通过核摄取胸腺嘧啶类似物5'-溴-2'-脱氧尿苷（BrdU）测定内皮细胞的有丝分裂活性（细胞增殖）。采用免疫印迹法测定内源性bFGF的含量。结果运动训练导致PAI受影响肌肉的功能增强，直到疲劳的跑步时间增加了近三倍。训练结扎肌的毛细血管细度明显大于静止结扎肌（P< 0.05）。运动引起的血管重塑包括新毛细血管的形成（血管生成），在研究的第3、5和7天内皮细胞增殖增强。然而，运动训练并没有改变闭塞肌肉中bFGF的含量。结论运动训练可改善PAI肌肉的功能和毛细血管。内皮细胞有丝分裂活性的增加证实了血管生成。然而，一种有效的血管生成因子bFGF的含量没有改变。因此，运动诱导的PAI受影响肌肉血管生成并不依赖于内源性bFGF表达的增加。
PURPOSEThis study was conducted to determine whether exercise-induced improvements in capillarity in muscle with peripheral arterial insufficiency (PAI) was accompanied by endothelial cell mitosis, and whether that response could be explained by changes in the expression of basic fibroblast growth factor (bFGF), a known mitogen.METHODSAfter bilateral ligation of femoral arteries, Sprague-Dawley rats either remained sedentary or participated in a treadmill running protocol. Running time to exhaustion at each session was recorded. On days 1, 2, 3, 5, and 7 of the experimental period, trained-ligated and sedentary-ligated animals were euthanized, and segments of muscle from the gastrocnemius were obtained. Capillarity was determined with histochemistry, and endothelial cell mitotic activity (cell proliferation) was assayed via nuclear uptake of 5'-bromo-2'-deoxyuridine (BrdU), an analog of thymidine. Content of endogenous bFGF was assessed with immunoblotting techniques.RESULTSExercise training resulted in augmented function of PAI affected muscle as evidenced by a nearly threefold increase in running time until exhaustion. Trained-ligated muscle demonstrated significantly (P< 0.05) greater capillarity than sedentary-ligated muscle. Vascular remodeling elicited by exercise included the formation of new capillaries (angiogenesis) as indicated by enhanced endothelial cell proliferation at days 3, 5, and 7 of the study. However, exercise training did not alter the content of bFGF in occluded muscle.CONCLUSIONIn muscle with PAI, exercise training improved functional capacity and capillarity. Angiogenesis was confirmed by the increased mitotic activity of endothelial cells. However, the content of bFGF, a potent angiogenic factor, was not altered. Thus, exercise-induced angiogenesis in PAI affected muscle is not dependent upon increased expression of endogenous bFGF.