ABP1 expression regulated by IAA and ABA is associated with the cambium periodicity in Eucommia ulmoides Oliv.
ABP1 expression regulated by IAA and ABA is associated with the cambium periodicity in Eucommia ulmoides Oliv.
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杜仲中 IAA 和 ABA 调节的 ABP1 表达与形成层周期性相关。
DOI：
10.1093/jxb/erl150
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发表时间：
2006-11-01
影响因子：
6.9
通讯作者：
Cui, Ke-Ming
中科院分区：
文献类型：
--
作者：
Hou, Hong-Wei;Zhou, Yu-Ting;Cui, Ke-Ming
杜仲的一个cDNA克隆。分离了生长素受体之一的生长素结合蛋白1(ABP1)基因，采用RT-PCR、实时定量PCR、Northern印迹、Western blotting和免疫定位等技术手段，研究了ABP1基因的季节性表达与IAA和ABA年变化的关系。在形成层区域，在形成层周期的活动期、静止期和休止期，ABP1在蛋白质和mRNA水平上的表达分别是高、低和极少的。ABP1的信号丰度遵循与ABA积累相反的模式，并与形成层组织的生长素反应相关，表明ABP1在活动-休眠周期中介导生长素依赖的形成层激活调节中发挥作用。这篇论文试图解释为什么IAA会‘促进’静止形成层的重新激活，而不是静止形成层的重新激活。结果还表明，IAA促进ABP1的表达，而ABA抑制ABP1的表达。
A cDNA clone of Eucommia ulmoides Oliv. encoding auxin binding protein 1 (ABP1), one of the putative receptors of auxin, was isolated, and the seasonal expression of ABP1 in relation to IAA and ABA annual variation was investigated by different technical approaches including RT-PCR, real-time PCR, northern blotting, western blotting, and immunolocalization. In the cambial region, ABP1 expression at both the protein and the mRNA level was found to be high, low, and remarkably scarce in the active, quiescent, and resting stages, respectively, during cambium periodicity. The signal abundance of ABP1 follows the opposite pattern to ABA accumulation and correlates with auxin responsiveness of the cambial tissues, suggesting a role for ABP1 in mediating auxin-dependent regulation of cambial activation in the activity-dormancy cycle. This paper attempts to explain why IAA would 'boost' the reactivation of a quiescent cambium, and not that of a resting cambium. Results also show that ABP1 expression is improved by IAA, while inhibited by ABA.