Peroxidase-based spectrophotometric methods for the determination of ascorbic acid, norepinephrine, epinephrine, dopamine and levodopa
Peroxidase-based spectrophotometric methods for the determination of ascorbic acid, norepinephrine, epinephrine, dopamine and levodopa
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DOI：
10.1016/s0003-2670(97)00561-8
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发表时间：
1997-12-31
影响因子：
6.2
通讯作者：
Shen, HX
中科院分区：
文献类型：
--
作者：
Zhu, M;Huang, XM;Shen, HX
建立了一种同时测定抗坏血酸、去甲肾上腺素、肾上腺素、多巴胺和左旋多巴的新方法。这是基于过氧化物酶活性的干扰，这导致对氯苯酚与4-氨基安替比林的偶联反应的速率降低。对干扰机理进行了初步研究，并确定了测定的最佳实验条件。对商业药物制剂的分析表明，与制造商给出的标称值以及相同样品的官方方法结果具有良好的相关性。(C)1997年Elsevier Science B.V.
A novel method was developed for the spectrophotometric determinations of ascorbic acid, norepinephrine, epinephrine, dopamine and levodopa. It is based on interference with peroxidase activity, which caused a decrease of the rate of the coupling reaction of p-chlorophenol with 4-aminoantipyrine. The mechanism of interference was studied in a preliminary way, and optimal experimental conditions for the determinations were established. Analysis of commercial pharmaceutical formulations showed excellent correlation with the nominal values given by the manufacturers and with the results of the official methods for the same samples. (C) 1997 Elsevier Science B.V.