Biocontrol activity of Paenibacillus polymyxa AC-1 against Pseudomonas syringae and its interaction with Arabidopsis thaliana
Biocontrol activity of Paenibacillus polymyxa AC-1 against Pseudomonas syringae and its interaction with Arabidopsis thaliana
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DOI：
10.1016/j.micres.2016.01.004
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发表时间：
2016-01-01
影响因子：
6.7
通讯作者：
Park, Jeong Mee
中科院分区：
文献类型：
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作者：
Hong, Chi Eun;Kwon, Suk Yoon;Park, Jeong Mee
多粘类芽孢杆菌AC-1 （Paenibacillus polymyxa AC-1，简称AC-1）是一种促进植物生长的根瘤菌（PGPR），已被用作防治植物病原真菌和促进植物生长的土壤接种剂。在这项研究中，我们研究了AC-1对植物病原菌丁香假单胞菌的影响，并通过计数定殖植物的细菌种群来研究AC-1对植物内部定殖的影响。AC-1抑制了紫丁香的生长。番茄DC3000 （Pst）和紫丁香p。烟粉虱（Pta）在体外测定中呈浓度依赖性。对异种生长的拟南芥根尖处滴入AC-1处理后，我们发现AC-1在侵染5天后大部分出现在拟南芥叶片内部而不是根部，表明AC-1发生了系统性传播。我们进一步研究了AC-1在缺乏植物激素信号通路的拟南芥突变体中的定殖模式。上述结果表明，脱落酸（ABA）和茉莉酸（JA）信号通路分别对AC-1在叶片中的定植有正向和负向作用，而对AC-1在根组织周围的附生积累没有影响。本研究表明AC-1是一种抑制丁香假单胞菌生长的有效生物防治剂，这可能与AC-1作为一种叶栖内生菌的定殖模式有关。本工作的结果将有助于扩大我们对AC-1作为生物防治剂的作用方式的理解，从而促进其在农业中的应用。(C) 2016 Elsevier GmbH版权所有。
Paenibacillus polymyxa AC-1 (AC-1) is a plant growth-promoting rhizobacterium (PGPR) that has been used as a soil inoculant for biocontrol of plant pathogenic fungi and to promote plant growth. In this study, we examine the effects of AC-1 on the bacterial phytopathogen Pseudomonas syringae and internal colonization of AC-1 by counting bacterial populations that colonize plants. AC-1 inhibited the growth of both P. syringae pv. tomato DC3000 (Pst) and P. syringae pv. tabaci (Pta) in a concentration-dependent manner in in vitro assays. Upon treatment of AC-1 dropping at root tip of axenically grown Arabidopsis, we found that most of the AC-1 was detected in interior of leaves of Arabidiopsis plants rather than roots after 5 days post infection, indicating systemic spreading of AC-1 occur. We examined further AC-1 colonization patterns in Arabidopsis mutants deficient in phytohormone signaling pathways. These results indicated that abscisic acid (ABA) and jasmonic acid (JA) signaling pathways positively and negatively contributed, respectively, to AC-1 colonization of leaves, whereas epiphytic accumulation of AC-1 around root tissues was not affected. This study shows that AC-1 is an effective biocontrol agent to suppress P. syringae growth, possibly owing to its colonization patterns as a leaf-inhabiting endophyte. The results showed in this work will help to expand our understanding of the mode of action of AC-1 as a biological control agent and consequently, its application in agriculture. (C) 2016 Elsevier GmbH. All rights reserved.