Distinct and complex bacterial profiles in human periodontitis and health revealed by 16S pyrosequencing
Distinct and complex bacterial profiles in human periodontitis and health revealed by 16S pyrosequencing
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DOI：
10.1038/ismej.2011.191
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发表时间：
2012-06-01
期刊：
影响因子：
11
通讯作者：
Leys, Eugene J.
中科院分区：
文献类型：
--
作者：
Griffen, Ann L.;Beall, Clifford J.;Leys, Eugene J.
牙周炎的病因是一个复杂的微生物生态系统。随着测序技术的进步，阐明细菌群落差异的全面研究最近已成为可能。我们使用454 16 S rRNA基因测序比较龈下细菌群落从29牙周健康对照组和29例慢性牙周炎。对16 S基因V1-2和V4区域的扩增子进行测序，产生1393579个序列。通过BLAST对口腔16 S数据库进行了鉴定，并映射到16门，106属和596种。81%的序列可定位于栽培种。在所有系统发育水平上观察到健康和牙周炎相关细菌群落之间的差异，UniFrac和主坐标分析显示健康和疾病的不同群落特征。群落多样性较高的疾病，并确定了123种，是显着更丰富的疾病，和53健康。螺旋体，Synergistetes和拟杆菌在疾病中更丰富，而变形菌在健康对照中的水平更高。在厚壁菌门中，芽孢杆菌类与健康相关，而梭菌属、阴性菌门和丹毒菌属与疾病相关。这些结果暗示了一些分类群，将成为未来研究的目标。一些，如Filifactoralocis和许多Spirochetes的代表了一个大部分的序列相比，以前确定的目标。阐明这些差异的社区组成提供了基础，进一步了解牙周炎的发病机制。The ISME Journal（2012）6，1176-1185; doi：10.1038/ismej.2011.191; 2011年12月15日在线发表
Periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. With advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. We used 454 sequencing of 16S rRNA genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. Amplicons from both the V1-2 and V4 regions of the 16S gene were sequenced, yielding 1 393 579 sequences. They were identified by BLAST against a curated oral 16S database, and mapped to 16 phyla, 106 genera, and 596 species. 81% of sequences could be mapped to cultivated species. Differences between health-and periodontitis-associated bacterial communities were observed at all phylogenetic levels, and UniFrac and principal coordinates analysis showed distinct community profiles in health and disease. Community diversity was higher in disease, and 123 species were identified that were significantly more abundant in disease, and 53 in health. Spirochaetes, Synergistetes and Bacteroidetes were more abundant in disease, whereas the Proteobacteria were found at higher levels in healthy controls. Within the phylum Firmicutes, the class Bacilli was health-associated, whereas the Clostridia, Negativicutes and Erysipelotrichia were associated with disease. These results implicate a number of taxa that will be targets for future research. Some, such as Filifactor alocis and many Spirochetes were represented by a large fraction of sequences as compared with previously identified targets. Elucidation of these differences in community composition provides a basis for further understanding the pathogenesis of periodontitis. The ISME Journal (2012) 6, 1176-1185; doi:10.1038/ismej.2011.191; published online 15 December 2011