RNA-DEPENDENT REPLICATION, TRANSCRIPTION, AND PERSISTENCE OF BROME MOSAIC-VIRUS RNA REPLICONS IN SACCHAROMYCES-CEREVISIAE
RNA-DEPENDENT REPLICATION, TRANSCRIPTION, AND PERSISTENCE OF BROME MOSAIC-VIRUS RNA REPLICONS IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0092-8674(93)90584-d
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发表时间：
1993-03-26
期刊：
影响因子：
64.5
通讯作者：
AHLQUIST, P
中科院分区：
文献类型：
--
作者：
JANDA, M;AHLQUIST, P
这篇论文表明酵母可以作为复制高等真核病毒基因组的宿主。雀麦花叶病毒(BMV)是动植物正链RNA病毒类α病毒超家族的一员。表达BMV RNA复制基因1a和2a的酵母支持依赖RNA的复制和BMV RNA3衍生物的转录。外壳基因被报告基因取代的RNA3衍生物的表达依赖于1a和2a在反式中的表达，以及BMV RNA复制和转录信号在顺式中的表达。携带酵母URA3基因的RNA3衍生物补充了ura3-酵母的原生营养，并以持久RNA异构体的形式存在。因此，BMV RNA复制和转录所必需的所有细胞因子都必须存在于酵母中，酵母遗传学应该有助于它们的鉴定。
This paper shows that yeast can be a host for the replication of a higher eukaryotic viral genome. Brome mosaic virus (BMV) is a member of the alphavirus-like superfamily of animal and plant positive strand RNA viruses. Yeast expressing BMV RNA replication genes 1a and 2a support RNA-dependent replication and transcription of BMV RNA3 derivatives. RNA3 derivatives with the coat gene replaced by a reporter gene expressed that gene in a fashion dependent on la and 2a expression in trans and on BMV RNA replication and transcription signals in cis. RNA3 derivatives carrying the yeast URA3 gene complemented ura3- yeast to prototrophy and were maintained as persistent RNA episomes. Thus, all cellular factors essential for BMV RNA replication and transcription must be present in yeast, and yeast genetics should facilitate their identification.