Bipolar silica nanochannel array for dual-mode electrochemiluminescence and electrochemical immunosensing platform
Bipolar silica nanochannel array for dual-mode electrochemiluminescence and electrochemical immunosensing platform
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用于双模式电化学发光和电化学免疫传感平台的双极性二氧化硅纳米通道阵列
DOI：
10.1016/j.snb.2022.132086
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发表时间：
2022-06-03
影响因子：
8.4
通讯作者：
Liu, Jiyang
中科院分区：
文献类型：
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作者：
Gong, Jiawei;Zhang, Tongtong;Liu, Jiyang
本文报道了利用双极性二氧化硅纳米通道阵列（bp-SNA）构建双模式电化学发光（ECL）和电化学（EC）传感平台，双功能探针（三（2，2 '-联吡啶）钌，Ru（bpy）32+）被限制在纳米通道内（命名为Ru@bp-SNA）。由于bp-SNA具有不对称的表面电荷，其在氧化铟锡电极上可以有效地作为静电纳米笼，通过内负表面的静电吸引和外正表面的静电排斥作用，稳定地限制Ru（bpy）32+。Ru（bpy）32+被固定到纳米空间中，作为高效的ECL发光体和定义明确的EC氧化还原分子来产生强信号。由于Ru@bp-SNA顶部的生物识别单元的修饰能够赋予优异的荧光能力并在特定分析物的存在下形成复合物，因此ECL和EC信号的降低使得能够定量检测该分析物。作为概念验证实验，已经分别通过ECL和EC模式成功地在水溶液和人血清中测定了两种肿瘤相关生物标志物CEA和CA 15 -3。
Herein we reported the construction of dual-mode electrochemiluminescence (ECL) and electrochemical (EC) sensing platform using the bipolar silica nanochannel array (bp-SNA) confined with the bifunctional probe (tris (2,2'-bipyridyl) ruthenium, Ru(bpy)32+) inside the nanochannels (designated as Ru@bp-SNA). With the asymmetric surface charges, bp-SNA on the indium tin oxide electrode can effectively function as electrostatic nanocage for the stable confinement of Ru(bpy)32+ via dual electrostatic force, namely electrostatic attraction of inner negative surface and electrostatic repulsion of outer positive surface. Ru(bpy)32+ immobilized into the nanospace acts as both highly efficient ECL luminophore and well-defined EC redox molecule to generate strong signal. Since the modification of bio-recognition units on the top of Ru@bp-SNA is able to confer excellent recognitive capacity and obtain the formation of complex in the presence of specific analyte, the decrease of ECL and EC signals enables the quantitative detection of this analyte. As proof-of-concept experiments, two tumor related biomarkers, CEA and CA15-3, have been successfully determined in aqueous solution and human serum by ECL and EC modes, respectively.