Antioxidant properties and principal phenolic phytochemicals of Indian medicinal plants from Asclepiadoideae and Periplocoideae
Antioxidant properties and principal phenolic phytochemicals of Indian medicinal plants from Asclepiadoideae and Periplocoideae
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DOI：
10.1080/14786410802228827
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发表时间：
2010-01-01
影响因子：
2.2
通讯作者：
Sun, Mei
中科院分区：
文献类型：
--
作者：
Surveswaran, Siddharthan;Cai, Yi-Zhong;Sun, Mei
夹竹桃亚科（夹竹桃科）和密切相关的Periplocoideae是许多印度本土药用植物的来源。我们调查了15个样品的抗氧化性能和总酚类和黄酮类化合物的含量，代表12个印度药用植物物种从这些亚科。采用2，2-连氮双-3-乙基苯并噻唑啉-6-磺酸法和铁还原抗氧化能力法进行总抗氧化剂测定。总酚和黄酮含量测定采用比色法。采用液相色谱-质谱联用技术（LC-MS）检测了主要酚类化合物。结果表明，黄花十鳞草叶片中总酚和黄酮含量最高，抗氧化能力最强。黄嘌呤氧化酶（XO）抑制活性以短柱肉茎最高。其中印度半带藻的根清除羟自由基的能力最强。总体而言，Periplocoideae成员比Asclepiadoideae成员具有更高的抗氧化活性。总抗氧化能力参数与总酚含量呈极显著正相关（R0.914），表明酚类化合物对供试植物样品的抗氧化活性有显著贡献。利用LC-MS技术鉴定了这些植物中主要的酚类化合物，包括黄酮类、酚酸类和酚萜类。绿原酸和芦丁在几乎所有的植物样品中被检测到。LC-MS分析提供了这两个亚科药用植物中主要酚类化合物的完整指纹图谱，可用于鉴别和质量评价。
The subfamily Asclepiadoideae (Apocynaceae) and the closely-related Periplocoideae are sources of many indigenous Indian medicinal plants. We surveyed antioxidant properties and total phenolic and flavonoid contents of 15 samples, representing 12 Indian medicinal plant species from these subfamilies. Total antioxidant assay was performed using the 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid and ferric-reducing antioxidant power methods. Total phenolic and flavonoid contents were measured using colourimetric methods. Principal phenolic compounds were detected by liquid chromatography-mass spectrometry (LC-MS). The highest antioxidant capacity and high levels of total phenolics and flavonoids were found in the leaves of Decalepis hamiltonii. The stems of Sarcostemma brevistigma exhibited the highest xanthine oxidase (XO) inhibitory activity. The roots of Hemidesmus indicus showed the highest OH- radical scavenging activity. In general, Periplocoideae members exhibited higher antioxidant activity than Asclepiadoideae members. The highly significant and positive correlations (R 0.914) between total antioxidant capacity parameters and total phenolic content indicated that the phenolic compounds contributed significantly to the antioxidant activity of the tested plant samples. The principal phenolic phytochemicals from these plants were identified by LC-MS, including flavonoids, phenolic acids and phenolic terpenoids. Chlorogenic acid and rutin were detected in almost all of the plant samples. The LC-MS analysis provided full fingerprints of the principal phenolic compounds in the medicinal plants from these two subfamilies, which are useful for their authentication and quality evaluation.