The effector SIX8 is required for virulence of Fusarium oxysporum f.sp. cubense tropical race 4 to Cavendish banana
The effector SIX8 is required for virulence of Fusarium oxysporum f.sp. cubense tropical race 4 to Cavendish banana
复制标题
尖镰刀菌 f.sp 的毒力需要效应子 SIX8。
DOI：
10.1016/j.funbio.2019.03.001
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发表时间：
2019-05-01
期刊：
影响因子：
2.5
通讯作者：
Wang, Qiannan
中科院分区：
文献类型：
--
作者：
An, Bang;Hou, Xingrong;Wang, Qiannan
植物病原菌利用效应子作为分子武器来操纵宿主免疫并促进定殖。香蕉枯萎香蕉枯萎病病原菌（sp. cubense）是香蕉幼苗枯萎病的病原菌，根据品种特异性鉴定出4个生理小种。在Foc TR 4的基因组中总共检测到9个SIX基因，在Foc 1中检测到6个基因。在这些SIX基因中，SIX 2和SIX 8仅在Foc TR 4中检测到，而在Foc 1中未鉴定到。表达谱分析表明，Foc TR 4的SIX基因在接种卡文迪什香蕉苗后被高度诱导。对SIX 2和SIX 8基因敲除突变体的毒力分析表明，SIX 8是Foc TR 4的毒力所必需的，而SIX 2没有明显的功能。SIX 8-FLAG蛋白在SIX 8敲除突变体中的过表达部分恢复了毒力。Western blot分析表明SIX 8可分泌到细胞外，其N端多肽序列含有信号肽。本研究为进一步研究SIX 8调控Foc TR 4毒力的机制提供了线索。(C)2019年英国真菌学会由爱思唯尔有限公司出版。保留所有权利。
Plant pathogens employ effectors as molecular weapons to manipulate host immunity and facilitate colonization. Fusarium oxysporum f. sp. cubense is the agent of wilt disease in banana plantlets and four races of the pathogen have been identified based on the cultivar specificity. A total of 9 SIX genes have been detected in the genome of Foc TR4 and 6 genes detected in Foc1. Among these SIX genes, SIX2 and SIX8 are only detected in Foc TR4, not identified in Foc1. Expression profiles analysis revealed that SIX genes of Foc TR4 are highly induced after inoculation to Cavendish banana plantlets. Virulence analysis of the SIX2 and SIX8 knock-out mutants showed that SIX8 is required for the virulence of Foc TR4 while SIX2 has no obvious functions. Over expression of SIX8-FLAG proteins in the SIX8 knock-out mutant partly restored the virulence. Western blot analysis suggested that SIX8 could be secreted into the extracellular space and a signal peptide resided the N-terminal polypeptide sequence. This study provides some clues for further research on mechanism of SIX8 in regulating virulence of Foc TR4. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.