Long pentraxin 3 (PTX3) expression and release by neutrophils in vitro and in ulcerative colitis
Long pentraxin 3 (PTX3) expression and release by neutrophils in vitro and in ulcerative colitis
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DOI：
10.1111/j.1440-1827.2011.02651.x
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发表时间：
2011-05-01
影响因子：
2.2
通讯作者：
Naito, Makoto
中科院分区：
文献类型：
--
作者：
Savchenko, Alexander S.;Inoue, Akira;Naito, Makoto
在培养的中性粒细胞中发现，在IL-8刺激下释放penttraxin 3蛋白，并在挤压DNA形成的中性粒细胞胞外陷阱（NETs）中定位乳铁蛋白+特异性颗粒。溃疡性结肠炎患者结肠黏膜组织中性粒细胞是PTX3蛋白的主要细胞来源，其表达与炎症的组织学分级有良好的相关性。抗乳铁蛋白抗体的免疫荧光分析显示，隐窝脓肿病变内中性粒细胞释放的NETs形成，通过IL-8受体B （CXCR2）的表达被激活。有趣的是，显示了PTX3蛋白缺失的中性粒细胞，支持隐窝脓肿中性粒细胞释放PTX3。我们怀疑PTX3蛋白可能有助于溃疡性结肠炎患者炎症结肠组织中细胞介导的免疫防御，特别是在隐窝脓肿病变中。
Pentraxin 3 protein was found to be present following release upon IL-8 stimulation in cultured neutrophils together with lactoferrin+-specific granules localized in neutrophil extracellular traps (NETs) formed by extruded DNA. Neutrophils in colonic mucosal tissue of patients with ulcerative colitis were the main cellular source of PTX3 protein, the expression of which is correlated well with the histological grades of inflammation. Immunofluorescence analysis against anti-lactoferrin antibody revealed the formation of NETs released from neutrophils within crypt abscess lesions, which were found to be activated through the expression of IL-8 receptor B (CXCR2). Of interest, neutrophils depleted of PTX3 protein were displayed, supporting the release of PTX3 from neutrophils in crypt abscess. We suspected that PTX3 protein may contribute to cell-mediated immune defense in inflamed colon tissue, and in particular in crypt abscess lesions, of patients with ulcerative colitis.