COMPARISON OF METHODS FOR THE DETERMINATION OF ABSOLUTE METABOLITE CONCENTRATIONS IN HUMAN MUSCLES BY P-31 MRS
COMPARISON OF METHODS FOR THE DETERMINATION OF ABSOLUTE METABOLITE CONCENTRATIONS IN HUMAN MUSCLES BY P-31 MRS
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DOI：
10.1002/mrm.1910300505
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发表时间：
1993-11-01
影响因子：
3.3
通讯作者：
BOESIGER, P
中科院分区：
文献类型：
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作者：
BUCHLI, R;BOESIGER, P
为了利用体内P-31 MRS测定人体骨骼肌中代谢物浓度，分析了不同定量方法对结果的准确性和重复性以及操作的简单性的影响。每种定量方法都包括校准策略和定位技术。大量的体内和体外试验表明，基于同核模型的校准策略比使用内水作为浓度标准的异核策略产生更准确（更低的系统误差）和更可重复（更低的统计误差）的浓度估计。此外，前一种策略比后一种策略更容易处理。体积选择序列ISIS的定位结果比表面线圈的定位结果重现性稍好。我们得出结论，基于幽灵的校准策略结合ISIS定位（测量误差几乎等于5-7%）可以最准确地测定磷代谢物浓度。
In order to determine metabolite concentrations in human skeletal muscles by in vivo P-31 MRS, different quantification methods were analyzed with regard to the accuracy and reproducibility of results and the simplicity of handling. Each quantification method comprised a calibration strategy and a localization technique. Extensive in vivo and in vitro tests showed that homonuclear phantom-based calibration strategies yielded significantly more accurate (lower systematic errors) and more reproducible (lower statistical errors) concentration estimates than heteronuclear strategies using internal water as a concentration standard. Additionally, the former strategies are easier to handle than the latter. Localization with the volume-selective sequence ISIS yielded slightly more reproducible results than localization by surface coil. We conclude that phosphorus metabolite concentrations are determined most accurately with phantom-based calibration strategies in combination with ISIS localization (measurement errors almost-equal-to 5-7%).