Transcript suppression of TaGW2 increased grain width and weight in bread wheat
Transcript suppression of TaGW2 increased grain width and weight in bread wheat
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DOI：
10.1007/s10142-014-0380-5
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发表时间：
2014-06-01
影响因子：
2.9
通讯作者：
Zhang, Zengyan
中科院分区：
文献类型：
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作者：
Hong, Yantao;Chen, Longfei;Zhang, Zengyan
面包小麦（Triticum aestivum L.）是世界上主要的粮食作物。粒重是决定小麦产量的主要因素，与粒重相关的候选基因的鉴定对小麦高产育种具有重要意义。TaGW 2是水稻OsGW 2的一个直向同源基因，负调控水稻粒宽和粒重。在面包小麦中存在三种TaGW 2同源物，TaGW 2A、TaGW 2B和TaGW 2D。本研究构建了TaGW 2-RNA干扰（RNAi）表达盒，并将其转化到中国小粒型面包小麦品种石4185中。TaGW 2-RNAi转基因小麦株系中TaGW 2A、TaGW 2B和TaGW 2D的转录水平同时下调。与对照相比，转TaGW 2基因小麦的粒宽和粒重显著增加，表明TaGW 2基因对小麦粒宽和粒重具有负调控作用。进一步的转录本分析表明，在不同的面包小麦材料中，TaGW 2A的转录本丰度与粒宽呈负相关。
Bread wheat (Triticum aestivum L.) is a major staple crop in the world. Grain weight is a major factor of grain yield in wheat, and the identification of candidate genes associated with grain weight is very important for high-yield breeding of wheat. TaGW2 is an orthologous gene of rice OsGW2 that negatively regulates the grain width and weight in rice. There are three TaGW2 homoeologs in bread wheat, TaGW2A, TaGW2B, and TaGW2D. In this study, a specific TaGW2-RNA interference (RNAi) cassette was constructed and transformed into a Chinese bread wheat variety 'Shi 4185' with small grain. The transcript levels of TaGW2A, TaGW2B, and TaGW2D were simultaneously downregulated in TaGW2-RNAi transgenic wheat lines. Compared with the controls, TaGW2-underexpressing transgenic lines displayed significantly increases in the grain width and weight, suggesting that TaGW2 negatively regulated the grain width and weight in bread wheat. Further transcript analysis showed that in different bread wheat accessions, the transcript abundance of TaGW2A was negatively associated with the grain width.