Differences in cyclin D2 and D1 protein expression distinguish forebrain progenitor subsets
Differences in cyclin D2 and D1 protein expression distinguish forebrain progenitor subsets
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DOI：
10.1093/cercor/bhk008
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发表时间：
2007-03-01
期刊：
影响因子：
3.7
通讯作者：
Ross, M. Elizabeth
中科院分区：
文献类型：
--
作者：
Glickstein, Sara B.;Alexander, Suzy;Ross, M. Elizabeth
神经增殖的调节是脑形成的重要组成部分，并且由内在细胞周期和外在生长和营养分子驱动。在细胞周期蛋白中，由于缺乏关于其表达模式的数据，对G1期活性细胞周期蛋白D2和D1（CD2和CD1）的相对作用的理解受到阻碍。在这项研究中，CD2免疫反应性检查在新皮质，神经节隆起/纹状体，海马结构从胚胎第12.5天，直到出生后60天，以更精确地表征这种蛋白质的表达在前脑发育。还对cD1的定位进行了免疫组织学定位以进行比较。在整个前脑发育过程中，这些细胞周期蛋白的重叠和非重叠蛋白表达表明存在区分祖细胞库的神经发生的共享和独特的细胞周期要求。
Regulation of neural proliferation is an essential component of brain formation and is driven by both intrinsic cell cycle and extrinsic growth and trophic molecules. Among the cell cycle proteins, understanding of the relative roles of the G1-phase active cyclins D2 and D1 (cD2 and cD1) has been hampered by lack of data regarding their expression patterns. In this study, cD2 immunoreactivity was examined in the neocortex, ganglionic eminences/striatum, and hippocampal formation from embryonic day 12.5 until postnatal day 60 to more precisely characterize the expression of this protein during forebrain development. The localization of cD1 was also immunohistologically mapped for comparison. Throughout forebrain development, both overlapping and nonoverlapping protein expression of these cyclins suggests the presence of shared and unique cell cycle requirements for neurogenesis that distinguishes progenitor pools.