Identification of G Protein-Coupled Receptors (GPCRs) Associated with Lambda-Cyhalothrin Detoxification in Cydia pomonella
Identification of G Protein-Coupled Receptors (GPCRs) Associated with Lambda-Cyhalothrin Detoxification in Cydia pomonella
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DOI：
10.1021/acs.jafc.3c06522
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发表时间：
2023-12-22
影响因子：
6.1
通讯作者：
Yang，Xue-Qing
中科院分区：
文献类型：
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作者：
Liu，Yu-Xi;Hu，Chao;Yang，Xue-Qing
虽然之前的研究报道了多种节肢动物中G蛋白偶联受体（GPCR）介导的杀虫剂抗性，但对单胞虫GPCR相关抗性机制的了解仍然有限。本研究共鉴定出4个家族的95个cpgpcr基因。结果显示，大多数cpgpcrs在第一幼虫期和头部的高表达水平。pomonella。暴露于氯氟氰菊酯显著增加了15CpGPCRs的表达，其中cpgpcr70在所有幼虫阶段均高表达，其中在中肠表达最高。RNA干扰（RNAi）表明，下调cpgpcr70可导致关键抗性相关基因的表达减少，导致幼虫对氯氟氰菊酯的耐受性降低。这些发现表明，cpgpcr70在调控抗氯氟氰菊酯解毒基因的表达中起着至关重要的作用，为制定更有效的害虫防治策略提供了有价值的见解。
While previous studies have reported G protein-coupled receptor (GPCR)-mediated insecticide resistance in various arthropods, the understanding of GPCR-associated resistance mechanisms inCydia pomonellaremains limited. In this study, a total of 95CpGPCRgenes categorized into four families were identified inC. pomonella. Results revealed high expression levels of the majority of theCpGPCRsduring the first larval stage and in the head ofC. pomonella.Exposure tolambda-cyhalothrin significantly increased the expression of 15CpGPCRs, includingCpGPCR70, which is highly expressed in all larval stages and shows the highest expression in the midgut. RNA interference (RNAi) demonstrated that downregulation ofCpGPCR70leads to reduced expression of key resistance-related genes and a decreased tolerance of larvae tolambda-cyhalothrin. These findings indicate thatCpGPCR70plays a crucial role in regulating the expression of detoxifying genes involved inlambda-cyhalothrin resistance, offering valuable insights for the development of more effective pest control strategies.