Creatine increases IGF-I and myogenic regulatory factor mRNA in C2C12 cells
Creatine increases IGF-I and myogenic regulatory factor mRNA in C2C12 cells
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DOI：
10.1016/s0014-5793(03)01504-7
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发表时间：
2004-01-16
期刊：
影响因子：
3.5
通讯作者：
Francaux, M
中科院分区：
文献类型：
--
作者：
Louis, M;Van Beneden, R;Francaux, M
向C2 C12细胞的分化培养基中添加肌酸导致肌管肥大。为了研究胰岛素样生长因子I（IGF-I）和生肌调节因子（MRF）在这种肥大中的作用，在分化的前72小时内评估了它们的mRNA水平。肌酸显着增加IGF-I mRNA水平在整个调查期间的时间，而MRF mRNA水平仅在精确的时刻增加，这表明IGF-I的一般激活机制和MRF转录的特异性调节机制。因此，我们的研究结果表明，肌酐诱导的C2 C12细胞肥大至少部分介导的IGF-I和MRF的过表达。(C)2003年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
Addition of creatine to the differentiation medium of C2C12 cells leads to hypertrophy of the myotubes. To investigate the implication of insulin-like growth factor I (IGF-I) and myogenic regulatory factors (MRFs) in this hypertrophy, their mRNA levels were assessed during the first 72 h of differentiation. Creatine significantly increased the IGF-I mRNA level over the whole investigated period of time, whereas the MRF mRNA levels were only augmented at precise moments, suggesting a general activation mechanism for IGF-I and a specifically regulated mechanism for MRF transcription. Our results suggest therefore that creatine-induced hypertrophy of C2C12 cells is at least partially mediated by overexpression of IGF-I and MRFs. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.