Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis
Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis
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DOI：
10.1101/gad.827500
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发表时间：
2000-09-15
影响因子：
10.5
通讯作者：
Choo, KHA
中科院分区：
文献类型：
--
作者：
Kalitsis, P;Earle, E;Choo, KHA
Bub 3是有丝分裂纺锤体组装复合体的保守组分。该蛋白质对于Bub 3基因破坏小鼠的早期发育至关重要，从它们在交配后6.5-7.5天（pc）后未能存活可见一斑。Bub 3无效胚胎在pc 3.5天内表现正常，但在pc 4.5-6.5天内以微核、染色质桥接、落后染色体和不规则核形态的形式积累有丝分裂错误。用纺锤体解聚剂处理的胚胎不能停滞在中期，并显示有丝分裂紊乱增加。结果证实Bub 3是在早期胚胎发生过程中起作用的基本纺锤体检查点通路的一个组成部分。
Bub3 is a conserved component of the mitotic spindle assembly complex. The protein is essential for early development in Bub3 gene-disrupted mice, evident from their failure to survive beyond day 6.5-7.5 postcoitus (pc). Bub3 null embryos appear normal up to day 3.5 pc but accumulate mitotic errors from days 4.5-6.5 pc in the form of micronuclei, chromatin bridging, lagging chromosomes, and irregular nuclear morphology. Null embryos treated with a spindle-depolymerising agent fail to arrest in metaphase and show an increase in mitotic disarray. The results confirm Bub3 as a component of the essential spindle checkpoint pathway that operates during early embryogenesis.