Increases in muscle MCT are associated with reductions in muscle lactate after a single exercise session in humans
Increases in muscle MCT are associated with reductions in muscle lactate after a single exercise session in humans
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DOI：
10.1152/ajpendo.2002.282.1.e154
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发表时间：
2002-01-01
影响因子：
5.1
通讯作者：
Ouyang, J
中科院分区：
文献类型：
--
作者：
Green, H;Halestrap, A;Ouyang, J
为了研究单次长周期运动[持续5-6 h，60%峰值O-2摄取（（V）/点O-2峰值）]对工作股外侧肌代谢适应的影响，在训练前（前）和训练后2（后2）、4（后4）和6（后6）天检查了9名未经训练的男性（峰值O-2摄取= 47.2 +/- 1.1ml.kg（-1）.min（-1），平均值+/- SE）。基于在点O-2峰上59%（V）的15分钟周期运动，发现与Pre（10.9 +/- 1.3）相比，训练在Post-2（6.65 +/- 0.69）、Post-4（7.74 +/- 0.63）和Post-6（7.78 +/- 1.2）降低（P< 0.05）运动肌肉乳酸（mM）。没有观察到训练对运动ATP、磷酸肌酸和糖原水平的影响。在单次训练后，血浆体积在2后（6.7 +/- 1.7%）、4后（5.86 +/- 1.9）和6后（5.13 +/- 2.5）升高（P< 0.05）。单次运动也导致运动后6天内单羧酸转运体MCT 1和MCT 4的升高（P< 0.05）。虽然肾上腺素和去甲肾上腺素都随着运动而增加，但只有去甲肾上腺素随着训练而减少（P< 0.05），并且仅在Post-4。这些结果表明，细胞乳酸水平的调节发生迅速，独立于其他代谢适应。有人提出，MCT和血浆容量的增加至少部分参与训练后观察到的较低的肌肉乳酸含量，通过增加乳酸膜运输和清除，分别。
To investigate the effects of a single session of prolonged cycle exercise [60% peak O-2 uptake ((V) over dot O-2peak) for 5-6 h] on metabolic adaptations in working vastus lateralis muscle, nine untrained males (peak O2 uptake = 47.2 +/- 1.1 ml.kg(-1).min(-1), means +/- SE) were examined before (Pre) and at 2 (Post-2), 4 (Post-4), and 6 (Post-6) days after the training session. On the basis of 15 min of cycle exercise at 59% (V) over dot O-2peak, it was found that training reduced (P< 0.05) exercise muscle lactate (mM) at Post-2 (6.65 +/- 0.69), Post-4 (7.74 +/- 0.63), and Post-6 (7.78 +/- 1.2) compared with Pre (10.9 +/- 1.3). No effect of training was observed on exercise ATP, phosphocreatine, and glycogen levels. After the single session of training, plasma volumes were elevated (P< 0.05) at Post-2 (6.7 +/- 1.7%), Post-4 (5.86 +/- 1.9), and Post-6 (5.13 +/- 2.5). The single exercise session also resulted in elevations (P< 0.05) in the monocarboxylate transporters MCT1 and MCT4 throughout the 6 days after exercise. Although epinephrine and norepinephrine both increased with exercise, only norepinephrine was reduced (P< 0.05) with training and only at Post-4. These results indicate that regulation of cellular lactate levels occurs rapidly and independently of other metabolic adaptations. It is proposed that increases in MCT and plasma volume are at least partly involved in the lower muscle lactate content observed after the training session by increasing lactate membrane transport and removal, respectively.