Glutathione S-transferase theta 1 expressed in granulosa cells as a biomarker for oocyte quality in age-related infertility
Glutathione S-transferase theta 1 expressed in granulosa cells as a biomarker for oocyte quality in age-related infertility
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DOI：
10.1016/j.fertnstert.2007.07.1389
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发表时间：
2008-10-01
影响因子：
6.7
通讯作者：
Saito, Hidekazu
中科院分区：
文献类型：
--
作者：
Ito, Megumu;Muraki, Miho;Saito, Hidekazu
目的：本研究的目的是确定一个可靠的生物标志物与年龄有关的不孕症。设计：实验室研究。设置：ART实验室。患者：接受卵胞浆内单精子注射或IVF周期的患者。干预措施：采用逆转录酶-聚合酶链反应（RT-PCR）技术检测了不孕患者卵泡壁细胞和卵丘颗粒细胞中谷胱甘肽S-转移酶（GST）mRNA和蛋白的表达。主要结果测量：颗粒细胞中GST θ 1（GSTT 1）表达与卵母细胞质量之间的相关性是主要结果测量。GSTT 1在男性因素患者颗粒细胞中的表达与年龄呈正相关，与卵丘-卵母细胞复合体成熟度呈负相关。当从其他不育因素中提取颗粒细胞中具有高和低GSTT 1的样品时，高GSTT 1组的卵丘-卵母细胞复合体成熟度显著低于低GSTT 1组（高：27.2% vs.低：51.3%）。高GSTT 1组卵母细胞的发育能力可能较低（高：26.4% vs.低：43.9%）。FSH和H2 O2可促进GSTT 1在增龄过程中的上调。结论：GSTT 1是一个良好的年龄相关性不孕的指标。
Objective: The goal of this study was to identify a reliable biomarker for age-related infertility.Design: Laboratory study.Setting: ART laboratory.Patient(s): Patients undergoing intracytoplasmic sperm injection or IVF cycles.Intervention(s): Expression of Glutathione S-transferase (GST) mRNA and protein in mural and cumulus granulosa cells obtained from infertile patients were examined by reverse transcriptase-polymerase chain reaction and immunofluorescence.Main Outcome Measure(s): Correlation between the expression of GST theta 1 (GSTT1) in granulosa cells and oocyte quality was a main outcome measure.Result(s): Expression of GSTT1 in granulosa cells from male factor patients was positively correlated with age and negatively with cumulus-oocyte complex maturity. When samples with high and low GSTT1 in granulosa cells were extracted from the other infertility factors, cumulus-oocyte complex maturity in the high GSTT1 group was significantly lower than that in the low GSTT1 group (high: 27.2% vs. low: 51.3%). The developmental capacity of oocytes in the high GSTT1 group was likely to be lower (high: 26.4% vs. low: 43.9%). Up-regulation of GSTT1 during aging may be promoted by FSH and H2O2, determined by an in vitro model.Conclusion(s): GSTT1 is a good indicator for age-related infertility.