Asiatic acid supplementation during the in vitro culture period improves early embryonic development of porcine embryos produced by parthenogenetic activation, somatic cell nuclear transfer and in vitro fertilization
Asiatic acid supplementation during the in vitro culture period improves early embryonic development of porcine embryos produced by parthenogenetic activation, somatic cell nuclear transfer and in vitro fertilization
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体外培养期间补充积雪草酸可改善孤雌激活、体细胞核移植和体外受精产生的猪胚胎的早期胚胎发育
DOI：
10.1016/j.theriogenology.2019.09.027
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发表时间：
2020
期刊：
影响因子：
2.8
通讯作者：
Sun Bo-Xing
中科院分区：
文献类型：
--
作者：
Qi Jia-Jia;Li Xiao Xia;Diao Yun Fei;Liu Peng-Lei;Wang Da-Li;Bai Chun-Yan;Yuan Bao;Liang Shuang;Sun Bo-Xing
积雪草酸是一种富含于中草药积雪草中的五环三萜类化合物，具有清除自由基和抗细胞凋亡的作用。本研究旨在探讨亚麻酸对猪早期胚胎发育的影响及其可能的作用机制。结果表明，在猪胚胎体外培养过程中添加10μ的亚麻酸，可显著提高猪孤雌激活、体细胞核移植和体外受精胚胎的发育能力。进一步分析表明，积雪草酸抑制了过氧化氢诱导的细胞内活性氧自由基(ROS)的产生。值得注意的是，亚麻酸不仅提高了细胞内的GSH水平，而且还减轻了线粒体功能障碍。基因表达分析显示，亚麻酸上调了抗氧化剂相关基因Sod-1和胚泡形成相关基因COX-2的表达，下调了凋亡相关基因Caspase-9的表达。这些结果表明，亚麻酸对猪胚胎的早期发育有一定的促进作用，可能有助于提高猪胚胎的体外生产。
Asiatic acid is a pentacyclic triterpene enriched in the medicinal herb Centella asiatica, and it has been suggested to possess free radical scavenging and anti-apoptotic properties. The purpose of the current study was to explore the effects of asiatic acid on porcine early-stage embryonic development and the potential mechanisms for any observed effects. The results showed that 10 μM asiatic acid supplementation during the in vitro culture period dramatically improved developmental competence in porcine embryos derived from parthenogenetic activation (PA), somatic cell nuclear transfer (SCNT) and in vitro fertilization (IVF). Further analysis revealed that asiatic acid attenuated H2O2-induced intracellular reactive oxygen species (ROS) generation. Notably, asiatic acid not only enhanced intracellular GSH levels but also attenuated mitochondrial dysfunction. Gene expression analysis revealed that asiatic acid upregulated expression of the antioxidant-related gene Sod-1 and the blastocyst formation related gene Cox-2, while downregulating expression of the apoptosis-related gene Caspase-9 in SCNT blastocysts. These results suggest that asiatic acid exerts beneficial effects on early embryonic development in porcine embryos and that asiatic acid may be useful for improving the in vitro production of porcine embryos.