Genetic characterization of the hemagglutinin genes of wild-type measles virus circulating in china, 1993-2009.
Genetic characterization of the hemagglutinin genes of wild-type measles virus circulating in china, 1993-2009.
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DOI：
10.1371/journal.pone.0073374
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Xu S;Zhang Y;Zhu Z;Liu C;Mao N;Ji Y;Wang H;Jiang X;Li C;Tang W;Feng D;Wang C;Zheng L;Lei Y;Ling H;Zhao C;Ma Y;He J;Wang Y;Li P;Guan R;Zhou S;Zhou J;Wang S;Zhang H;Zheng H;Liu L;Ma H;Guan J;Lu P;Feng Y;Zhang Y;Zhou S;Xiong Y;Ba Z;Chen H;Yang X;Bo F;Ma Y;Liang Y;Lei Y;Gu S;Liu W;Chen M;Featherstone D;Jee Y;Bellini WJ;Rota PA;Xu W
中国在过去20年中经历了几次大规模麻疹暴发，并实施了一系列加强控制措施，以实现消除麻疹的目标。对野生型麻疹病毒(MeV)的分子流行病学监测提供了有关病毒传播模式的宝贵信息。自1993年以来，病毒学监测证实，中国流行的是单一流行的H1型病毒。分子监测的一个组成部分是监测MeV血凝素(H)基因的遗传特征，该基因是病毒中和抗体的主要目标。对1993年-2009年中国流行的56株甲型脑炎病毒代表野毒株的H基因全序列分析表明，H基因序列被聚为2类，即类1和类2，其中类1株是检出频率最高的类群，2000年后在中国有广泛的分布。预测的H蛋白氨基酸序列在大多数有功能意义的氨基酸位置上相对保守。然而，大多数H1型Cluster1病毒有一个氨基酸替换(Ser240Asn)，它移除了一个预测的N-连接糖基化位点。此外，在56株菌株中有23株在血凝素套索表位(HNE)上检测到Pro397Leu的替代。H1型病毒H基因的进化速率估计为每年每个位点约0.76×10−3个替换，dN/ds(dN/ds)比值为-lt；1，表明没有选择压力。虽然H1型毒株的H基因是保守的，不受选择压力的影响，但在功能重要的位置上观察到了几个氨基酸替换。因此，应将野生型MEVS H基因的抗原性和遗传特性作为中国麻疹常规分子监测的一部分。
China experienced several large measles outbreaks in the past two decades, and a series of enhanced control measures were implemented to achieve the goal of measles elimination. Molecular epidemiologic surveillance of wild-type measles viruses (MeV) provides valuable information about the viral transmission patterns. Since 1993, virologic surveillnace has confirmed that a single endemic genotype H1 viruses have been predominantly circulating in China. A component of molecular surveillance is to monitor the genetic characteristics of the hemagglutinin (H) gene of MeV, the major target for virus neutralizing antibodies. Analysis of the sequences of the complete H gene from 56 representative wild-type MeV strains circulating in China during 1993–2009 showed that the H gene sequences were clustered into 2 groups, cluster 1 and cluster 2. Cluster1 strains were the most frequently detected cluster and had a widespread distribution in China after 2000. The predicted amino acid sequences of the H protein were relatively conserved at most of the functionally significant amino acid positions. However, most of the genotype H1 cluster1 viruses had an amino acid substitution (Ser240Asn), which removed a predicted N-linked glycosylation site. In addition, the substitution of Pro397Leu in the hemagglutinin noose epitope (HNE) was identified in 23 of 56 strains. The evolutionary rate of the H gene of the genotype H1 viruses was estimated to be approximately 0.76×10−3 substitutions per site per year, and the ratio of dN to dS (dN/dS) was <1 indicating the absence of selective pressure. Although H genes of the genotype H1 strains were conserved and not subjected to selective pressure, several amino acid substitutions were observed in functionally important positions. Therefore the antigenic and genetic properties of H genes of wild-type MeVs should be monitored as part of routine molecular surveillance for measles in China.
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通讯作者：
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