Copying of RNA Sequences without Pre-Activation.
Copying of RNA Sequences without Pre-Activation.
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DOI：
10.1002/anie.201506592
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发表时间：
2015-11-23
期刊：
影响因子：
--
通讯作者：
Richert C
中科院分区：
文献类型：
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作者：
Jauker M;Griesser H;Richert C
核苷酸在不断生长的互补链末端的模板定向结合是复制的基础。对于RNA，如果核糖核苷酸首先转化为具有离去基的活性物质，则该过程可以在没有酶的情况下发生。到目前为止，激活需要一个单独的化学步骤，使益生元的可能性变得复杂。在这里，我们证明了碳二亚胺和有机催化剂的组合诱导了四种核糖核苷酸中的任何一种的近定量结合。在原位激活后，发现单磷酸腺苷也在水溶液中形成低聚物。因此，从头开始的链形成和序列特异性复制都可以在没有人工合成步骤的情况下发生。
Template-directed incorporation of nucleotides at the terminus of a growing complementary strand is the basis of replication. For RNA, this process can occur in the absence of enzymes, if the ribonucleotides are first converted to an active species with a leaving group. Thus far, the activation required a separate chemical step, complicating prebiotically plausible scenarios. Here we show that a combination of a carbodiimide and an organocatalyst induces near-quantitative incorporation of any of the four ribonucleotides. Upon in situ activation, adenosine monophosphate was found to also form oligomers in aqueous solution. So, both de novo strand formation and sequence-specific copying can occur without an artificial synthetic step.