Heterogeneity of human lymphokine (IL‐2)‐activated killer (LAK) precursors and regulation of their LAK induction by blood monocytes
Heterogeneity of human lymphokine (IL‐2)‐activated killer (LAK) precursors and regulation of their LAK induction by blood monocytes
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人淋巴因子 (IL-2) 激活的杀伤细胞 (LAK) 前体的异质性及其血液单核细胞对 LAK 诱导的调节
DOI：
10.1002/ijc.2910420320
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发表时间：
1988
影响因子：
6.4
通讯作者：
T. Ogura
中科院分区：
文献类型：
--
作者：
S. Sone;N. Inamura;A. Nii;T. Ogura
用CCE法从健康献血者外周血中分离出高纯度淋巴细胞（> 99%）和单核细胞（>90%）。血液淋巴细胞经CCE分离为9个亚群。这些淋巴细胞组分对NK敏感的K - 562细胞的NK活性，以及对NK细胞抗性靶细胞（Daudi）的LAK活性，在有或没有最佳IL - 2浓度的情况下孵育4天后进行检测。4小时51Cr释放法测定NK和LAK活性。根据NK和LAK活性，将这些淋巴细胞分为3个LAK前体亚群：一个缺乏NK和LAK活性（Fr. 2），一个具有中等NK活性但LAK活性低（Fr. 5），一个同时具有NK和LAK活性（Fr. 8）。在淋巴细胞培养物中加入新鲜的自体单核细胞，可显著提高LAK在Fr. 2和Fr. 5中的活性。在平行实验中，通过测量淋巴细胞对IL - 2的成母反应，单核细胞对Fr. 2和Fr. 5的上调得到了证实。Fr. 8中CD16+ (Leu‐11+)NK细胞的淋巴细胞耗损导致LAK诱导减少74%，而Fr. 2中与CD3+ (OKT3+)抗体反应的细胞的单核细胞和淋巴细胞混合物耗损导致LAK诱导减少66%。用4系人肺癌细胞作为LAK活性的靶点，证实了单核细胞对LAK前体诱导LAK细胞的上调。这些结果清楚地表明，人单核细胞可能导致IL - 2 - in诱导的LAK活性在T细胞和NK细胞亚群中的表达上调。
Highly purified lymphocytes (> 99%) and monocytes (>90%) were isolated by CCE from peripheral blood of healthy donors. Blood lymphocytes were separated by this CCE into 9 subpopulations. The NK activities of these lymphocyte fractions against NK‐sensitive K‐562 cells and their LAK activities against NK cell‐resistant target (Daudi) cells were assayed promptly or after incubation of the fractions for 4 days with or without an optimal concentration of IL‐2. NK and LAK activities were measured by 4‐hr 51Cr‐release assay. On the basis of their NK and LAK activities, these lymphocyte fractions were classified into 3 subpopulations of LAK precursors: one lacking both NK and LAK activities (Fr. 2), one with moderate NK activity but low LAK activity (Fr. 5), and one possessing both NK and LAK activities (Fr. 8). Addition of autologous fresh monocytes to the lymphocyte cultures resulted in a significant increase in induction of LAK activity in Fr. 2 and Fr. 5. This up‐regulation of lymphocytes in Fr. 2 and Fr. 5 by monocytes was confirmed in parallel experiments by measuring the blastogenic response of the lymphocytes to IL‐2. Depletion of lymphocytes in Fr. 8 of CD16+ (Leu‐11+) NK cells resulted in 74% reduction in LAK induction, whereas depletion of mixtures of monocytes and lymphocytes in Fr. 2 of cells reacting with CD3+ (OKT3+) antibody resulted in a 66% reduction in LAK induction. This up‐regulation of LAK cell induction from LAK precursors by monocytes was confirmed using 4 lines of human lung cancer cells as targets for LAK activity. These results clearly indicate that human monocytes may cause up‐regulation of the expression of IL‐2‐in‐duced LAK activity in T cells and in a subpopulation of NK cells.
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HNK-1 (Leu-7) 人淋巴细胞的表征。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Abo,T;Cooper,MD;Balch,CM
通讯作者：
Balch,CM
人淋巴因子激活杀伤（LAK）细胞：两种类型效应细胞的鉴定。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Tilden,AB;Itoh,K;Balch,CM
通讯作者：
Balch,CM
重组白细胞介素 2 增强了表达 Leu 7 和 Leu 11 抗原的人类淋巴细胞亚群中自然杀伤细胞介导的细胞毒性。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lanier,LL;Benike,CJ;Phillips,JH;Engleman,EG
通讯作者：
Engleman,EG
由抗原受体特异性单克隆抗体诱导的克隆辅助 T 细胞系的生长：白细胞介素 1 受体的表达需要白细胞介素 2 受体。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kaye,J;Gillis,S;Mizel,SB;Shevach,EM;Malek,TR;Dinarello,CA;Lachman,LB;JanewayJr,CA
通讯作者：
JanewayJr,CA