Changes in membrane fatty acids composition of microbial cells induced by addiction of thymol, carvacrol, limonene, cinnamaldehyde, and eugenol in the growing media
Changes in membrane fatty acids composition of microbial cells induced by addiction of thymol, carvacrol, limonene, cinnamaldehyde, and eugenol in the growing media
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DOI：
10.1021/jf052722l
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发表时间：
2006-04-05
影响因子：
6.1
通讯作者：
Mauriello, G
中科院分区：
文献类型：
--
作者：
Di Pasqua, R;Hoskins, N;Mauriello, G
众所周知，精油中的主要活性化合物具有抗病原体和腐败微生物的抗微生物活性。这项工作的目的是确定膜脂肪酸谱的改变作为细胞在亚致死浓度的抗微生物化合物存在下响应于应激条件的适应机制。将麝香草酚、香芹酚、柠檬烯、肉桂醛和丁香酚的甲醇溶液加入到大肠杆菌O157：H7、沙门氏菌鼠伤寒血清型、荧光假单胞菌、嗜热短丝菌和金黄色葡萄球菌菌株的生长培养基中。进行脂肪酸提取和气相色谱分析，以评估膜脂肪酸组成的变化。当E.大肠杆菌和沙门氏菌菌株分别在柠檬烯和肉桂醛以及香芹酚和丁香酚的存在下生长。所有化合物均影响B的脂肪酸谱。Thermoacidacta，而假单胞菌和S.金黄色葡萄球菌菌株在它们的脂肪酸组成中没有显示出实质性的变化。
Major active compounds from essential oils are well-known to possess antimicrobial activity against both pathogen and spoilage microorganisms. The aim of this work was to determine the alteration of the membrane fatty acid profile as an adaptive mechanism of the cells in the presence of a sublethal concentration of antimicrobial compound in response to a stress condition. Methanolic solutions of thymol, carvacrol, limonene, cinnamaldehyde, and eugenol were added into growth media of Escherichia coli O157:H7, Salmonella enterica serovar typhimurium, Pseudomonas fluorescens, Brochothrix thermosphacta, and Staphylococcus aureus strains. Fatty acid extraction and gas chromatographic analysis were performed to assess changes in membrane fatty acid composition. Substantial changes were observed on the long chain unsaturated fatty acids when the E. coli and Salmonella strains grew in the presence of limonene and cinnamaldehyde and carvacrol and eugenol, respectively. All compounds influenced the fatty acid profile of B. thermosphacta, while Pseudomonas and S. aureus strains did not show substantial changes in their fatty acid compositions.