The impact of supplemental carbon sources on Arabidopsis thaliana growth, chlorophyll content and anthocyanin accumulation
The impact of supplemental carbon sources on Arabidopsis thaliana growth, chlorophyll content and anthocyanin accumulation
复制标题
DOI：
10.1007/s10725-009-9412-x
复制
发表时间：
2009-12-01
影响因子：
4.2
通讯作者：
Harrington, Gregory N.
中科院分区：
文献类型：
--
作者：
Stevenson, Carrie C.;Harrington, Gregory N.
本研究探讨了多种碳源对拟南芥生长发育的影响。测定的参数包括暗萌发下胚轴长度、光萌发根生长、莲座丛生长、叶绿素浓度和花青素含量。处理糖包括蔗糖、麦芽糖、d-葡萄糖、d-果糖、l-阿拉伯糖、l-岩藻糖、d-半乳糖、d-甘露糖、l-鼠李糖和d-木糖，每种糖以4、20或100 mM供应。根系生长对补充碳源特别敏感。在100 mM蔗糖、麦芽糖、葡萄糖或木糖上的生长刺激根生长接近100%。生长在阿拉伯糖，岩藻糖，半乳糖，甘露糖或鼠李糖抑制根生长50%或更多。强烈抑制根生长的几种糖对下胚轴伸长和莲座丛生长没有影响（半乳糖和岩藻糖）或有积极影响（阿拉伯糖）。鼠李糖是在所有浓度下抑制下胚轴伸长的唯一碳源。蔗糖、麦芽糖、葡萄糖、果糖、阿拉伯糖或木糖刺激莲座丛生长的幅度接近50%。叶绿素含量强烈降低甘露糖，而蔗糖，葡萄糖，半乳糖和鼠李糖造成较小的减少。半乳糖和甘露糖均强烈诱导花色素苷的积累。在所有浓度下，仅甘露糖影响所有参数。基于这些数据，可以得出结论，每种碳源对拟南芥生长和发育的影响在幅度和影响的参数方面都是特定的。
This research explores the impacts of a broad range of supplemental carbon sources on growth and development of Arabidopsis thaliana. Parameters measured include dark-germinated hypocotyl length, light-germinated root growth, rosette growth, chlorophyll concentration and anthocyanin content. Treatment sugars include sucrose, maltose, d-glucose, d-fructose, l-arabinose, l-fucose, d-galactose, d-mannose, l-rhamnose and d-xylose each supplied at 4, 20 or 100 mM. This comparison of the effect of different carbon sources on multiple parameters and under identical conditions showed that every carbon source had unique qualitative and quantitative effects on Arabidopsis growth and development. Root growth was particularly sensitive to supplemental carbon source. Growth on 100 mM sucrose, maltose, glucose or xylose stimulated root growth by similar to 100%. Growth on arabinose, fucose, galactose, mannose or rhamnose inhibited root growth by 50% or more. Several sugars that strongly inhibited root growth had either no effect (galactose and fucose) or a positive effect (arabinose) on hypocotyl elongation and rosette growth. Rhamnose was the only carbon source that inhibited hypocotyl elongation across all concentrations. Sucrose, maltose, glucose, fructose, arabinose or xylose stimulated rosette growth by similar to 50%. Chlorophyll content was strongly reduced by mannose while sucrose, glucose, galactose and rhamnose caused smaller reductions. Anthocyanin accumulation was strongly induced by both galactose and mannose. Only mannose impacted all parameters across all concentrations. Based on these data it can be concluded that the effect of each carbon source on Arabidopsis growth and development is specific in terms of both magnitude and the parameters impacted.