Prevalence of Plasmid-mediated Colistin Resistance Gene mcr-1 in Domestic Wastewater
Prevalence of Plasmid-mediated Colistin Resistance Gene mcr-1 in Domestic Wastewater
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DOI：
10.1088/1755-1315/496/1/012015
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发表时间：
2020-06
期刊：
影响因子：
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通讯作者：
T. Vu;I. Kasuga
中科院分区：
文献类型：
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作者：
T. Vu;I. Kasuga
粘菌素是最后的抗生素之一。然而，由mcr-1编码的可传播质粒介导的粘菌素耐药基因于2015年在中国首次被检测到。mcr-1的传播受到极大关注。本研究调查了越南和日本城市污水中mcr-1的患病率，因为生活污水反映了城市居民的健康状况。于2019年9月从越南河内的To巫妖河和Nhue河采集水样。这些河流受到未经处理的生活污水的严重污染，因此，这些样品可以被视为城市污水。我们还于2019年10月从日本三个不同的城市污水处理厂（A、B和C）收集了废水样本。从样本中提取DNA，并通过定量PCR分析mcr-1丰度。定量PCR结果显示，所有样本中均检出mcr-1，表明健康人群中存在携带mcr-1的细菌。A污水处理厂进水中mcr-1的丰度（1.9 × 106基因拷贝/L）低于日本和越南的其他样品（8.1 × 106 ~ 1.3 × 107基因拷贝/L）。mcr-1在废水处理中的对数减少值范围为1.5-4.2 log 10，导致最终流出物中的丰度为7.0 × 102-7.3 × 104拷贝/L。越南和日本城市污水中的mcr-1水平相当，表明了可传播粘菌素耐药性的全球传播。废水被认为是mcr-1的重要监测目标。
Colistin is one of the last-resort antibiotics. However, the transmissible plasmid-mediated colistin resistance gene encoded by mcr-1 was first detected in China in 2015. The dissemination of mcr-1 is of great concern. This study investigates the prevalence of mcr-1 in urban sewage in Vietnam and Japan because domestic sewage reflects the health status of urban citizens. Water samples were collected from To Lich River and Nhue River in Hanoi, Vietnam, in September 2019. These rivers are severely polluted by untreated domestic wastewater; thus, these samples can be regarded as urban sewage. We also collected wastewater samples from three different municipal wastewater treatment plants (A, B, and C) in Japan in October 2019. DNA was extracted from the samples, and mcr-1 abundance was analyzed via quantitative PCR. Quantitative PCR demonstrated that mcr-1 was detected in all samples, indicating that healthy citizens carry some bacteria harboring mcr-1. The abundance of mcr-1 in the influent of wastewater treatment plant A (1.9 × 106 gene copies/L) was lower than that in the other samples in Japan and Vietnam (8.1 × 106–1.3 × 107 gene copies/L). The log reduction values of mcr-1 in wastewater treatment ranged 1.5–4.2 log10, resulting in abundance of 7.0 × 102–7.3 × 104 copies/L in the final effluents. The comparable levels of mcr-1 in urban sewage in Vietnam and Japan indicate the global spread of transmissible colistin resistance. Wastewater is considered as an important monitoring target for mcr-1.