Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages.
Neural crest cells retain their capability for multipotential differentiation even after lineage-restricted stages.
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即使在谱系限制阶段之后，神经嵴细胞仍保留其多向分化的能力。
DOI：
10.1002/dvdy.22658
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Takahiro Kunisada.
中科院分区：
文献类型：
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作者：
Tsutomu Motohashi;Katsumasa Yamanaka;Kairi Chiba;Kentaro Miyajima;Hitomi Aoki;Tomohisa Hirobe;Takahiro Kunisada.
神经嵴细胞（NC 细胞）的多能性被认为是其产生早期的一个过渡阶段； NC 细胞从神经管中出现后，被指定为谱系限制性前体细胞。我们分析了源自 Sox10-IRES-Venus ES 细胞的早期 NC 样细胞的分化，其中 Sox10 的表达可以通过荧光蛋白可视化。出乎意料的是，在体内分别限制为神经元（N）-胶质细胞（G）谱系和黑素细胞（M）谱系的Sox10+/Kit−细胞和Sox10+/Kit+细胞均产生了N、G和M，表明它们保留了多能性。我们用 Sox10-IRES-Venus ES 细胞培育小鼠并分析其 NC 细胞的分化。从这些小鼠中分离出的 Sox10+/Kit− 细胞和 Sox10+/Kit+ 细胞均形成含有 N、G 和 M 的集落，表明它们也是多能的。这些发现表明，即使在最初的谱系限制阶段之后，NC 细胞仍保留多能性。发展动力学 240:1681–1693, 2011。© 2011 Wiley-Liss, Inc.
Multipotency of neural crest cells (NC cells) is thought to be a transient phase at the early stage of their generation; after NC cells emerge from the neural tube, they are specified into the lineage‐restricted precursors. We analyzed the differentiation of early‐stage NC‐like cells derived from Sox10‐IRES‐Venus ES cells, where the expression of Sox10 can be visualized with a fluorescent protein. Unexpectedly, both the Sox10+/Kit− cells and the Sox10+/Kit+ cells, which were restricted in vivo to the neuron (N)‐glial cell (G) lineage and melanocyte (M) lineage, respectively, generated N, G, and M, showing that they retain multipotency. We generated mice from the Sox10‐IRES‐Venus ES cells and analyzed the differentiation of their NC cells. Both the Sox10+/Kit− cells and Sox10+/Kit+ cells isolated from these mice formed colonies containing N, G, and M, showing that they are also multipotent. These findings suggest that NC cells retain multipotency even after the initial lineage‐restricted stages. Developmental Dynamics 240:1681–1693, 2011. © 2011 Wiley‐Liss, Inc.