Diversity in protein profiles of individual calcium oxalate kidney stones.
Diversity in protein profiles of individual calcium oxalate kidney stones.
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DOI：
10.1371/journal.pone.0068624
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Takao T
中科院分区：
文献类型：
--
作者：
Okumura N;Tsujihata M;Momohara C;Yoshioka I;Suto K;Nonomura N;Okuyama A;Takao T
草酸钙肾结石含有少量的蛋白质，其中一些与肾结石的进展或预防有关。为了深入了解尿石症的病理生理学，我们用蛋白质组学的方法研究了草酸钙肾结石的蛋白质成分。据报道，从肾结石中提取的蛋白质在凝胶电泳中显示出高度的异质性迁移模式。这可能主要是由于Tamm-Horsfall蛋白和凝血酶原的蛋白质降解和蛋白质-蛋白质交联所致。采用溶液中蛋白酶消化-纳米LC-MALDI-串联质谱仪获得草酸钙肾结石的蛋白质组分，共鉴定出9例尿石症患者结石中的92种蛋白质。进一步的分析表明，蛋白质的种类和相对数量在不同的石头之间有很大的差异。虽然大多数结石中含有凝血酶原、骨桥蛋白、钙颗粒蛋白A和钙颗粒蛋白B等蛋白质，但也有一些样品的凝血酶原和骨桥蛋白含量较高，而另一些样品的钙颗粒蛋白含量较高。此外，富含钙颗粒的结石含有各种中性粒细胞丰富的蛋白，如髓过氧化物酶和乳转铁蛋白。这些个体肾结石的蛋白质组图谱表明，由不同蛋白质组组成的多个系统，包括白细胞来源的蛋白质，在个体肾结石的发病机制中所涉及的不同情况视情况而定。
Calcium oxalate kidney stones contain low amounts of proteins, some of which have been implicated in progression or prevention of kidney stone formation. To gain insights into the pathophysiology of urolithiasis, we have characterized protein components of calcium oxalate kidney stones by proteomic approaches. Proteins extracted from kidney stones showed highly heterogeneous migration patterns in gel electrophoresis as reported. This was likely to be mainly due to proteolytic degradation and protein-protein crosslinking of Tamm-Horsfall protein and prothrombin. Protein profiles of calcium oxalate kidney stones were obtained by in-solution protease digestion followed by nanoLC-MALDI-tandem mass spectrometry, which resulted in identification of a total of 92 proteins in stones from 9 urolithiasis patients. Further analysis showed that protein species and their relative amounts were highly variable among individual stones. Although proteins such as prothrombin, osteopontin, calgranulin A and calgranulin B were found in most stones tested, some samples had high contents of prothrombin and osteopontin, while others had high contents of calgranulins. In addition, calgranulin-rich stones had various neutrophil-enriched proteins such as myeloperoxidase and lactotransferrin. These proteomic profiles of individual kidney stones suggest that multiple systems composed of different groups of proteins including leucocyte-derived ones are differently involved in pathogenesis of individual kidney stones depending on situations.
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DOI：
10.1152/ajprenal.00134.2008
发表时间：
2008-10-01
影响因子：
4.2
作者：
Merchant, Michael L.;Cummins, Timothy D.;Lederer, Eleanor D.
通讯作者：
Lederer, Eleanor D.
影响因子：
5
作者：
Mushtaq, Shamim;Siddiqui, Anwar Ali;Shafqat, Jawed
通讯作者：
Shafqat, Jawed
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
影响因子：
5.9
作者：
BOYCE, WH
通讯作者：
BOYCE, WH
影响因子：
--
作者：
HESS, B
通讯作者：
HESS, B