Real-time tracking of hydrogen peroxide secreted by live cells using MnO2 nanoparticles intercalated layered doubled hydroxide nanohybrids
Real-time tracking of hydrogen peroxide secreted by live cells using MnO2 nanoparticles intercalated layered doubled hydroxide nanohybrids
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DOI：
10.1016/j.aca.2015.09.053
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发表时间：
2015-10-22
影响因子：
6.2
通讯作者：
Liu, Hongfang
中科院分区：
文献类型：
--
作者：
Asif, Muhammad;Aziz, Ayesha;Liu, Hongfang
本文报道了一种简便、绿色的基于MnO2纳米颗粒结合MgAl LDH p型半导体通道制备新型电催化剂的方法，并探讨了其作为电化学生物传感器高性能电极材料的实际应用。采用简易共沉淀法制备了固定Mg/Al （M2+/M3+原子比为3）和不同MnCl2中心点4H(2)O量的MgAl层状双氢氧化物（LDH）纳米杂化体。这种方法展示了LDH的不同特性，包括LDH包埋纳米粒子的良好嵌入特性和LDH宿主层中MnO2纳米粒子的高负载。在所有样品中，负载0.04%锰的Mn5-MgAl具有良好的结晶形貌。将分散良好的MnO2纳米颗粒包裹在水滑石基质中，由于MnO2良好的催化能力和导电MgAl LDH的协同作用，MnO2纳米颗粒对H2O2还原的电催化活性增强，并且具有优异的稳定性、选择性和可重复性。Mn5-MgAl修饰的玻璃碳电极（GCE）线性范围为0.05 ~ 78 mM，最低检出限为5 μ M (S/N = 3)，检测灵敏度为0.9352 μ M-1。这种优异的性能使其能够用于实时跟踪活HeLa细胞分泌的H2O2。这项工作可能为临床诊断、现场环境分析和护理点检测设备提供新的见解。(C) 2015年Elsevier B.V.出版
We report a facile and green method for the fabrication of new type of electrocatalysts based on MnO2 nanoparticles incorporated on MgAl LDH P-type semiconductive channel and explore its practical applications as high-performance electrode materials for electrochemical biosensor. A series of MgAl layered doubled hydroxide (LDH) nanohybrids with fixed Mg/Al (M2+/M3+ atomic ratio of 3) and varied amount of MnCl2 center dot 4H(2)O are fabricated by a facile co-precipitation method. This approach demonstrates the combination of distinct properties including excellent intercalation features of LDH for entrapping nanoparticles and high loading of MnO2 nanoparticles in the host layers of LDH. Among all samples, Mn5-MgAl with 0.04% loaded manganese has a good crystalline morphology. A well-dispersed MnO2 nanoparticles encapsulated into the host matrix of hydrotalcite exhibit enhanced electrocatalytic activity towards the reduction of H2O2 as well as excellent stability, selectivity and reproducibility due to synergistic effect of good catalytic ability of MnO2 and conductive MgAl LDH. Glass carbon electrode (GCE) modified with Mn5-MgAl possesses a wide linear range of 0.05-78 mM, lowest detection limit 5 mu M (S/N = 3) and detection sensitivity of 0.9352 mu AmM-1. This outstanding performance enables it to be used for real-time tracking of H2O2 secreted by live HeLa cells. This work may provide new insight in clinical diagnosis, on-site environmental analysis and point of care testing devices. (C) 2015 Published by Elsevier B.V.