Arabidopsis thaliana SHAGGY-related protein kinases (AtSK11 and 12) function in perianth and gynoecium development
Arabidopsis thaliana SHAGGY-related protein kinases (AtSK11 and 12) function in perianth and gynoecium development
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DOI：
10.1046/j.1365-313x.2000.00691.x
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发表时间：
2000-03-01
期刊：
影响因子：
7.2
通讯作者：
Kreis, M
中科院分区：
文献类型：
--
作者：
Dornelas, MC;van Lammeren, AAM;Kreis, M
在高等植物中，花分生组织在器官类型、数量和形式方面的正确模式是基因网络协调表达的结果。我们描述了由于拟南芥蓬蓬相关蛋白激酶基因AtSK11（ASK α）和AtSK12（ASK γ）转录水平降低而导致的花图案表型。AtSK基因是果蝇（Drosophila shaggy）基因和哺乳动物糖原合成酶激酶3 （GSK-3）的植物同源基因。SGG蛋白激酶是无翼信号通路的关键组成部分，是建立组织模式和细胞命运决定所必需的。通过原位杂交检测到的AtSK11（ASK α）和AtSK12（ASK γ）基因在野生型拟南芥花序发育过程中的表达模式，已被证明与花分生组织模式的可能作用一致。AtSK11（ASK α）和AtSK12（ASK γ）转录本在花序分生组织外围和花分生组织中检测到。在后期，AtSK基因的表达被定位于发育中的花器官原基的特定区域。此外，我们获得并分析了含有AtSK11（ASK α）和AtSK12（ASK γ）基因特异性反义结构的转基因植物。这些植物发育的花显示出更多的花被器官和雌蕊的顶基模式的改变。
In higher plants, the correct patterning of the floral meristem in terms of organ type, number and form is the result of a concerted expression of a network of genes. We describe phenotypes of flower patterning, resulting from a reduction of transcript levels of the Arabidopsis SHAGGY-related protein kinase genes AtSK11(ASK alpha) and AtSK12(ASK gamma). The AtSK genes are plant homologues of the Drosophila shaggy (SGG) gene and the mammalian Glycogen-Synthase Kinase-3 (GSK-3). The SGG protein kinase is a key component of the wingless signalling pathway and is required for the establishment of tissue patterning and cell fate determination. The expression patterns of the AtSK11(ASK alpha) and AtSK12(ASK gamma) genes during wild-type Arabidopsis inflorescence development, detected by in situ hybridisation, have been shown to be consistent with a possible role in floral meristem patterning. AtSK11(ASK alpha) and AtSK12(ASK gamma) transcripts were detected at the periphery of the inflorescence meristem and in the floral meristem. At later stages the expression of the AtSK genes became localised in specific regions of developing flower organ primordia. Furthermore, we have obtained and analysed transgenic plants containing AtSK11(ASK alpha) and AtSK12(ASK gamma) gene specific antisense constructs. These plants developed flowers showing a higher number of perianth organs and an alteration of the apical-basal patterning of the gynoecium.