The biogenesis of the Golgi ribbon: The roles of membrane input from the ER and of GM130
The biogenesis of the Golgi ribbon: The roles of membrane input from the ER and of GM130
复制标题
DOI：
10.1091/mbc.e06-10-0886
复制
发表时间：
2007-05-01
影响因子：
3.3
通讯作者：
De Matteis, Maria Antonietta
中科院分区：
文献类型：
--
作者：
Marra, Pierfrancesco;Salvatore, Lorena;De Matteis, Maria Antonietta
哺乳动物细胞中的高尔基复合体形成了一条由相互连接的扁平池堆叠而成的连续带状结构。我们在这里表明，这种独特的架构反映和需要从内质网（ER）的膜的连续输入，在多形ER-高尔基载体（EGCs）的形式。高尔基带生物发生的一个重要步骤是EGCs完全整合到堆栈中。这需要高尔基体基质蛋白GM 130，其在顺式高尔基体隔室和EGCs之间连续循环。在获得GM 130时，EGCs经历同型系留和融合，成熟为更大和更均匀的膜单元，其似乎准备并入高尔基体堆叠。在缺乏GM 130的情况下，这一过程受到损害，EGCs仍然是不同的实体。这会导致管泡膜的积累、池的缩短和高尔基带的断裂。然而，在这些条件下，分泌货物仍然可以交付给高尔基复合体，虽然这发生效率较低，显然是通过短暂的和/或有限的连续性之间的EGCs和高尔基池。
The Golgi complex in mammalian cells forms a continuous ribbon of interconnected stacks of flat cisternae. We show here that this distinctive architecture reflects and requires the continuous input of membranes from the endoplasmic reticulum (ER), in the form of pleiomorphic ER-to-Golgi carriers (EGCs). An important step in the biogenesis of the Golgi ribbon is the complete incorporation of the EGCs into the stacks. This requires the Golgi-matrix protein GM130, which continuously cycles between the cis-Golgi compartments and the EGCs. On acquiring GM130, the EGCs undergo homotypic tethering and fusion, maturing into larger and more homogeneous membrane units that appear primed for incorporation into the Golgi stacks. In the absence of GM130, this process is impaired and the EGCs remain as distinct entities. This induces the accumulation of tubulovesicular membranes, the shortening of the cisternae, and the breakdown of the Golgi ribbon. Under these conditions, however, secretory cargo can still be delivered to the Golgi complex, although this occurs less efficiently, and apparently through transient and/or limited continuities between the EGCs and the Golgi cisternae.