Effect of electrical stimulation of the nucleus of the solitary tract on the development of electrical amygdaloid kindling in the cat
Effect of electrical stimulation of the nucleus of the solitary tract on the development of electrical amygdaloid kindling in the cat
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DOI：
10.1046/j.1528-1157.2002.05702.x
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发表时间：
2002-09-01
期刊：
影响因子：
5.6
通讯作者：
Fernández-Guardiola, A
中科院分区：
文献类型：
--
作者：
Magdaleno-Madrigal, VM;Valdés-Cruz, A;Fernández-Guardiola, A
目的：本研究分析了电刺激孤束核 (NTS) 对自由活动的猫杏仁核电点燃 (AK) 发育的影响。方法：使用 9 只雄性成年猫，其两侧杏仁核（基底外侧核）、两侧膝状体、左侧 NTS 和两侧前额皮质均植入电极。还记录肌电图和眼电图。每 24 小时执行一次 AK（1 秒序列、1 毫秒脉冲、60 Hz、300600 muA）。之前在上午 10:00 AK 之前刺激 NTS 1 分钟（0.5 毫秒脉冲，30 Hz，150-300 muA），然后从上午 11:00 到下午 2:00 每 60 分钟刺激 4 次。在不同的日子里，暂停所有NTS刺激，并继续AK，直到达到VI点燃阶段。结果：NTS刺激产生的行为变化是眨眼、不动期、向上视线。舔、吞咽。同时刺激 NTS 和 AK 的动物没有达到 VI 阶段，仍处于行为阶段 I-III。故意暂停 NTS 刺激后，达到 VI 阶段，振幅。持续时间和杏仁核后放电的传播在 NTS 刺激期间没有表现出渐进进化。仅进行 AK 刺激的天数与同时进行 NTS 刺激的天数之间进行回归分析。显示出正相关（r 值 = 0.84）。结论：我们的结果表明 NTS 刺激干扰了惊厥进化和二次泛化的发展。这种延迟效应可能是由于蓝斑和中脑网状结构的某些区域的激活所致。除其他结构外，已被证明可以抑制实验性惊厥发作。
Purpose: This work analyzed the effect of electrical stimulation of the nucleus of the solitary tract (NTS) on the development of electrical amygdaloid kindling (AK) in freely moving cats.Methods: Nine male adult cats with implanted electrodes in both amygdalae (basolateral nucleus), both lateral geniculate bodies, left NTS, and both prefrontal cortices were used. Electromyogram and electrooculogram also were recorded. The AK was performed every 24 h (1-s train, 1-ms pulses, 60 Hz, 300600 muA). The NTS was stimulated previously for I min (0.5-ms pulses, 30 Hz, 150-300 muA), just before the AK at 10:00 a.m., and then every 60 min, 4 times, from 11:00 a.m. to 2:00 p.m. On different days, all NTS stimulation was suspended, and AK was continued until stage VI kindling was reached.Results: Behavioral changes produced by the stimulation of the NTS were blinking, immobility periods with upward sight. licking, and swallowing. Animals with simultaneous stimulation of NTS and AK did not reach stage VI remaining in behavioral stages I-III. Stage VI was reached after NTS stimulation was intentionally suspended, The amplitude. duration, and the propagation of the amygdaloid afterdischarge did not exhibit progressive evolution during NTS Stimulation. A regression analysis performed between the number of days with only AK stimulation and days with simultaneous NTS stimulation. which showed a positive correlation (values of r = 0.84).Conclusions: Our results suggest that NTS stimulation interferes with the development Of convulsive evolution and secondary generalization. This delay effect may be due to the activation of the locus ceruleus and some area,, of the midbrain reticular formation. among other structures, which has been demonstrated to inhibit experimental convulsive seizures.