Identification and Functional Characterization of a Maize Phosphate Transporter Induced by Mycorrhiza Formation
Identification and Functional Characterization of a Maize Phosphate Transporter Induced by Mycorrhiza Formation
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菌根形成诱导的玉米磷酸转运蛋白的鉴定和功能表征
DOI：
10.1093/pcp/pcy094
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发表时间：
2018-08-01
影响因子：
4.9
通讯作者：
Cheng, Beijiu
中科院分区：
文献类型：
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作者：
Liu, Fang;Xu, Yunjian;Cheng, Beijiu
磷（P）是植物生命所必需的大量营养素，尽管它通常不容易被作物所利用。丛枝菌根真菌（AMF）可以通过诱导某些磷转运蛋白的表达来提高植物体内的磷水平。磷转运蛋白对共生磷的吸收是AMF定殖和丛枝动态的关键。然而，菌根诱导的玉米Pi转运蛋白的功能在很大程度上尚不清楚。我们主要研究了磷浓度与AMF在玉米中定殖之间的相互作用，并检测了磷转运蛋白的诱导。研究了高磷和低磷条件下玉米丛枝菌根真菌的定殖和丛枝丛的发育。低磷增加了丛枝菌根的定殖，促进了丛枝的发育。磷浓度的进一步测定表明，AMF显著改善了低磷条件下玉米的磷营养状况。在这里，我们确定了Pi转运蛋白基因，ZmPt9，这是由菌根形成诱导。此外，ZmPt9过表达的根难以被AMF定殖。Pi响应分析表明，ZmPt9补充了Pi转运活性缺陷的酵母突变体，并提高了水稻中的P浓度。总之，这些数据表明，ZmPt9是一个菌根诱导的Pi转运蛋白基因参与Pi的吸收。
Phosphorus (P) is an essential macronutrient for plant life, although it is frequently not readily available to crops. Arbuscular mycorrhiza fungi (AMF) can improve plant P levels by inducing the expression of some phosphate (Pi) transporters. Symbiotic Pi uptake by Pi transporters is crucial for AMF colonization and arbuscule dynamics. However, the functions of mycorrhiza-inducible maize Pi transporters are largely unclear. We focused on the interaction between the Pi concentration and AMF colonization in maize, and detecting the induction of a Pi transporter. We investigated AMF colonization and arbuscular development in maize under high and low Pi environments. Low Pi increased AMF colonization and promoted arbuscular development. Further measurement of P concentration showed that AMF significantly improved the maize P status under low Pi conditions. Here, we identified the Pi transporter gene, ZmPt9, which was induced by mycorrhiza formation. In addition, ZmPt9-overexpressing roots were difficult to colonize by AMF. Pi response analysis showed that ZmPt9 complements a yeast mutant defective in Pi transporter activity and improves the P concentration in rice. Together, these data indicated that ZmPt9 is a mycorrhiza-inducible Pi transporter gene involved in Pi uptake.