The effect of acetaminophen on oxidative modification of low-density lipoproteins in hypercholesterolemic rabbits
The effect of acetaminophen on oxidative modification of low-density lipoproteins in hypercholesterolemic rabbits
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DOI：
10.3164/jcbn.2007004
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发表时间：
2007-07-01
影响因子：
2.4
通讯作者：
Pabuccuoglu, Aysun
中科院分区：
文献类型：
--
作者：
Ozsoy, Meral Bas;Pabuccuoglu, Aysun
低密度脂蛋白（LDL）的氧化修饰有助于动脉粥样硬化的病理学。抗氧化剂可以保护低密度脂蛋白免受氧化修饰。对乙酰氨基酚是一种广泛使用的镇痛解热剂，具有显着的抗氧化特性。然而，几乎没有证据表明乙酰氨基酚可以作为体内 LDL 氧化的抗氧化剂。在这项研究中，我们研究了对乙酰氨基酚对高胆固醇血症兔体内 LDL 氧化的影响。 LDL 的氧化修饰通过共轭二烯和硫代巴比妥酸反应物质 (TBARS) 进行鉴定。饲喂含1%g胆固醇饲料8周的胆固醇组，血浆和分离的LDL样本中TBARS含量和共轭二烯水平与对照兔相比显着升高（P < 0.05）。然而，在胆固醇+对乙酰氨基酚组中，TBARS含量和共轭二烯水平显着低于胆固醇组(p < 0.05)。体外研究的结果还表明，从血清中分离的 LDL 被 Cu++ 离子氧化，并且这种氧化在对乙酰氨基酚的存在下被还原。对乙酰氨基酚减少低密度脂蛋白的氧化修饰可能对预防动脉粥样硬化的发生和进展具有治疗价值。
Oxidative modification of low-density lipoproteins (LDL) contributes to the pathology of atherosclerosis. Antioxidants may protect LDL against oxidative modification. Acetaminophen, a widely used analgesic and antipyretic agent, has significant antioxidant properties. However, there is little evidence to suggest that acetarninophen acts as an antioxidant for LDL oxidation in vivo. In this study, we investigated the in vivo effect of acetaminophen on LDL oxidation in hypercholesterolemic rabbits. The oxidative modification of LDL was identified by conjugated dienes and thiobarbituric acid-reactive substances (TBARS). In the cholesterol group which rabbits were fed a diet contained 1% g cholesterol for 8 weeks, TBARS contents and conjugated diene levels in the plasma and isolated LDL samples significantly increased compared with the control rabbits (P < 0.05). However, in the cholesterol + acetaminophen group, the TBARS contents and conjugated diene levels were significantly lower than that of the cholesterol group (p < 0.05). The results from in vitro studies also demonstrated that the LDL isolated from serum was oxidized by Cu++ ions and this oxidation reduced in the presence of acetaminophen. The reduced oxidative modification of LDL by acetaminophen may be of therapeutic value in preventing the development and progression of atherosclerosis.