Non-Invasive imaging of extracellular vesicles: Quo vaditis in vivo?
Non-Invasive imaging of extracellular vesicles: Quo vaditis in vivo?
复制标题
细胞外囊泡的非侵入性成像：在体内将何去何从？
DOI：
10.1002/jev2.12241
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发表时间：
2022-07
影响因子：
16
通讯作者：
Bulte, Jeff W. M.
中科院分区：
文献类型：
--
作者：
Arifin, Dian R.;Witwer, Kenneth W.;Bulte, Jeff W. M.
细胞外囊泡（EVs）是由几乎所有细胞类型释放的脂质双层限定的囊泡，作为细胞间信号传导的介质。最近的证据表明，EVs在许多正常以及病理细胞过程中发挥关键作用。EVs可被用作疾病生物标志物，也可作为靶向的、无细胞的治疗递送和信号传导载体，用于再生医学和其他临床环境。尽管有这种潜力，但在将EVs施用于活体受试者后，其在体内的生物分布和药代动力学特征仍有很多未知之处。对体外源EVs进行非侵入性成像的能力，特别是在较大动物中，将有助于更好地了解它们在体内的归巢和滞留模式、血液和组织半衰期以及排泄途径，所有这些对于推进EVs的临床诊断和/或治疗应用都是必需的。我们介绍了用各种诊断造影剂和示踪剂标记EVs的当前最先进方法，以及可用于其体内可视化的相应成像方式：磁共振成像（MRI）、X射线计算机断层扫描（CT）成像、磁性粒子成像（MPI）、单光子发射计算机断层扫描（SPECT）、正电子发射断层扫描（PET）和光学成像（荧光和生物发光成像）。我们在此回顾了每种EV成像方法的优缺点，特别强调了临床转化。
Extracellular vesicles (EVs) are lipid‐bilayer delimited vesicles released by nearly all cell types that serve as mediators of intercellular signalling. Recent evidence has shown that EVs play a key role in many normal as well as pathological cellular processes. EVs can be exploited as disease biomarkers and also as targeted, cell‐free therapeutic delivery and signalling vehicles for use in regenerative medicine and other clinical settings. Despite this potential, much remains unknown about the in vivo biodistribution and pharmacokinetic profiles of EVs after administration into living subjects. The ability to non‐invasively image exogeneous EVs, especially in larger animals, will allow a better understanding of their in vivo homing and retention patterns, blood and tissue half‐life, and excretion pathways, all of which are needed to advance clinical diagnostic and/or therapeutic applications of EVs. We present the current state‐of‐the‐art methods for labeling EVs with various diagnostic contrast agents and tracers and the respective imaging modalities that can be used for their in vivo visualization: magnetic resonance imaging (MRI), X‐ray computed tomography (CT) imaging, magnetic particle imaging (MPI), single‐photon emission computed tomography (SPECT), positron emission tomography (PET), and optical imaging (fluorescence and bioluminescence imaging). We review here the strengths and weaknesses of each of these EV imaging approaches, with special emphasis on clinical translation.
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DOI：
10.1038/mt.2008.1
发表时间：
2008-04
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
Dai S;Wei D;Wu Z;Zhou X;Wei X;Huang H;Li G
通讯作者：
Li G
影响因子：
46.9
作者：
Alvarez-Erviti, Lydia;Seow, Yiqi;Wood, Matthew J. A.
通讯作者：
Wood, Matthew J. A.
影响因子：
8
作者：
Busato A;Bonafede R;Bontempi P;Scambi I;Schiaffino L;Benati D;Malatesta M;Sbarbati A;Marzola P;Mariotti R
通讯作者：
Mariotti R
影响因子：
17.1
作者：
Bose RJC;Uday Kumar S;Zeng Y;Afjei R;Robinson E;Lau K;Bermudez A;Habte F;Pitteri SJ;Sinclair R;Willmann JK;Massoud TF;Gambhir SS;Paulmurugan R
通讯作者：
Paulmurugan R
影响因子：
3.4
作者：
Dominkus, Pia Puzar;Stenovec, Matjaz;Lenassi, Metka
通讯作者：
Lenassi, Metka