Solution NMR studies of apo-mSin3A and-mSin3B reveal that the PAH1 and PAH2 domains are structurally independent
Solution NMR studies of apo-mSin3A and-mSin3B reveal that the PAH1 and PAH2 domains are structurally independent
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DOI：
10.1110/ps.073097308
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发表时间：
2008-01-01
期刊：
影响因子：
8
通讯作者：
Radhakrishnan, Ishwar
中科院分区：
文献类型：
--
作者：
He, Yuan;Radhakrishnan, Ishwar
进化上保守的哺乳动物Sin 3（mSin 3）转录辅阻遏物主要通过位于N端附近的两个PAH（成对两亲性螺旋）结构域与多种转录因子相互作用。以前的研究表明，可能的域间相互作用涉及PAH域。在这里，我们表明，结构域是结构上独立的和个别域的属性，如构象的异质性和mSin 3A PAH 2的同源二聚化的能力，被保存在跨越两个PAH域的结构。因此，我们的研究结果表明，Sin 3蛋白的N-末端片段广泛可用于与其他蛋白质的相互作用，PAH结构域被组织成结构独立的模块。我们的数据还排除了旁系同源mSin 3A和mSin 3B蛋白之间通过涉及mSin 3A PAH 2结构域的相互作用的任何异型关联。
The evolutionarily conserved mammalian Sin3 (mSin3) transcriptional corepressor interacts with a diverse array of transcription factors mainly through two PAH (paired amphipathic helix) domains located near the N terminus. Previous studies suggested the possibility of interdomain interactions involving the PAH domains. Here, we show that the domains are structurally independent and the properties of the individual domains, such as the conformational heterogeneity and the ability of mSin3A PAH2 to homodimerize, are preserved in constructs that span both PAH domains. Our results thus suggest that the N-terminal segments of the Sin3 proteins are broadly available for interactions with other proteins and that the PAH domains are organized into structurally independent modules. Our data also rule out any heterotypic association between the paralogous mSin3A and mSin3B proteins via interactions involving the mSin3A PAH2 domain.