EFFECT OF HYDROGEN-PEROXIDE ON RUBIDIUM TRANSPORT OF RAT LENS
EFFECT OF HYDROGEN-PEROXIDE ON RUBIDIUM TRANSPORT OF RAT LENS
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DOI：
10.1016/0014-4835(76)90217-7
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发表时间：
1976-01-01
影响因子：
3.4
通讯作者：
FUKUI, HN
中科院分区：
文献类型：
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作者：
FUKUI, HN
进一步探讨了抗坏血酸和H2O2（抗坏血酸氧化的产物）影响晶状体阳离子运输机制的可能性。由于抗坏血酸对晶状体阳离子运输的影响可能主要是由H2O2引起的，因此我们用过氧化物进行了进一步的研究。当晶状体在含H2O2但不含葡萄糖的培养基中孵育时，Rb+的摄取受到不利影响。因此，过氧化物具有与抗坏血酸类似的作用。在培养基中加入葡萄糖可以消除H2O2和抗坏血酸的作用。H2O2降低了晶状体的谷胱甘肽水平，而抗坏血酸则没有。
The possibilities of ascorbic acid and H2O2, a product of ascorbate oxidation, influencing the cation transport mechanism in the lens were further explored. Since the possibility that the ascorbate effect on the cation transport of the lens may be largely due to the H2O2, further studies were made with peroxide. When the lens was incubated in a medium containing H2O2 but not glucose, the uptake of Rb+ was adversely affected. Thus peroxide had similar effects as ascorbate. The addition of glucose to the medium abolished both the H2O2 and the ascorbate effect. H2O2 lowered the glutathione level of the lens whereas ascorbate did not.